SKAP1 is dispensable for chemokine induced; migration of primary T-cells. Export

@article{citeulike:6076182, abstract = {Immune adaptors SLP-76, ADAP and SKAP1 (SKAP-55) play central roles in anti-CD3 induced 'inside-out' signalling for LFA-1 activation and ICAM-1 adhesion. However, it has been unclear whether SKAP1 is also required for chemokine-induced T-cell motility. In this study, we found that SDF-1 and CCL21 induced similar motility in SKAP1 deficient (SKAP1-/-) and wild type (SKAP1+/+) resting, primary T-cells. In addition, the speed (i.e. 13muM/min), tracking distance (i.e. length) and displacement values (i.e. direct distance between the start and the end positions of cell movement) in response to SDF1 were similar for SKAP1-/- and SKAP1+/+ primary, actvated T-cells. Anti-CD3 ligation also arrested the migration (i.e. stop-signal) of resting SKAP1+/+ and SKAP1-/- T-cells in the presence of SDF-1 and CCL21. These data demonstrate that contrary to its central role in anti-CD3 induced LFA-1 adhesion, the response of primary T-cells to SDF-1 and CCL21 is not profoundly dependent on SKAP1 expression.}, author = {Wang, Hongyan and Lu, Yuning and Rudd, Christopher E.}, citeulike-article-id = {6076182}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.imlet.2009.10.010}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19883688}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19883688}, day = {30}, doi = {10.1016/j.imlet.2009.10.010}, issn = {1879-0542}, journal = {Immunology letters}, keywords = {chemokine, migration, skap55}, month = {October}, posted-at = {2009-11-05 19:14:19}, priority = {2}, title = {SKAP1 is dispensable for chemokine induced; migration of primary T-cells.}, url = {http://dx.doi.org/10.1016/j.imlet.2009.10.010}, year = {2009} }

TY - JOUR ID - citeulike:6076182 L3 - citeulike-article-id:6076182 N2 - Immune adaptors SLP-76, ADAP and SKAP1 (SKAP-55) play central roles in anti-CD3 induced 'inside-out' signalling for LFA-1 activation and ICAM-1 adhesion. However, it has been unclear whether SKAP1 is also required for chemokine-induced T-cell motility. In this study, we found that SDF-1 and CCL21 induced similar motility in SKAP1 deficient (SKAP1-/-) and wild type (SKAP1+/+) resting, primary T-cells. In addition, the speed (i.e. 13muM/min), tracking distance (i.e. length) and displacement values (i.e. direct distance between the start and the end positions of cell movement) in response to SDF1 were similar for SKAP1-/- and SKAP1+/+ primary, actvated T-cells. Anti-CD3 ligation also arrested the migration (i.e. stop-signal) of resting SKAP1+/+ and SKAP1-/- T-cells in the presence of SDF-1 and CCL21. These data demonstrate that contrary to its central role in anti-CD3 induced LFA-1 adhesion, the response of primary T-cells to SDF-1 and CCL21 is not profoundly dependent on SKAP1 expression. JF - Immunology letters SN - 1879-0542 TI - SKAP1 is dispensable for chemokine induced; migration of primary T-cells. KW - chemokine KW - migration KW - skap55 AU - Wang, Hongyan AU - Lu, Yuning AU - Rudd, Christopher PY - 2009/10/30/ UR - http://dx.doi.org/10.1016/j.imlet.2009.10.010 ER -