Targeting Plasmodium ligands on mosquito salivary glands and midgut with a phage display peptide library. Export

@article{citeulike:2194343, abstract = {Despite vast efforts and expenditures in the past few decades, malaria continues to kill millions of persons every year, and new approaches for disease control are urgently needed. To complete its life cycle in the mosquito, Plasmodium, the causative agent of malaria, has to traverse the epithelia of the midgut and salivary glands. Although strong circumstantial evidence indicates that parasite interactions with the two organs are specific, hardly any information is available about the interacting molecules. By use of a phage display library, we identified a 12-aa peptide--salivary gland and midgut peptide 1 (SM1)--that binds to the distal lobes of the salivary gland and to the luminal side of the midgut epithelium, but not to the midgut surface facing the hemolymph or to ovaries. The coincidence of the tissues with which parasites and the SM1 peptide interact suggested that the parasite and peptide recognize the same surface ligand. In support of this hypothesis, the SM1 peptide strongly inhibited Plasmodium invasion of salivary gland and midgut epithelia. These experiments suggest a new strategy for the genetic manipulation of mosquito vectorial capacity.}, address = {Department of Genetics, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106-4955, USA.}, author = {Ghosh, A. K. and Ribolla, P. E. and Jacobs-Lorena, M.}, citeulike-article-id = {2194343}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.241491198}, citeulike-linkout-1 = {http://www.pnas.org/content/98/23/13278.full.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/98/23/13278.full.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/11687659}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=11687659}, day = {6}, doi = {10.1073/pnas.241491198}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, keywords = {anti\_malarial\_peptides, malaria, phage\_display, plasmodium, screen, sm1}, month = {November}, number = {23}, pages = {13278--13281}, posted-at = {2009-05-16 21:59:09}, priority = {2}, title = {Targeting Plasmodium ligands on mosquito salivary glands and midgut with a phage display peptide library.}, url = {http://dx.doi.org/10.1073/pnas.241491198}, volume = {98}, year = {2001} }

TY - JOUR ID - citeulike:2194343 L3 - citeulike-article-id:2194343 N2 - Despite vast efforts and expenditures in the past few decades, malaria continues to kill millions of persons every year, and new approaches for disease control are urgently needed. To complete its life cycle in the mosquito, Plasmodium, the causative agent of malaria, has to traverse the epithelia of the midgut and salivary glands. Although strong circumstantial evidence indicates that parasite interactions with the two organs are specific, hardly any information is available about the interacting molecules. By use of a phage display library, we identified a 12-aa peptide--salivary gland and midgut peptide 1 (SM1)--that binds to the distal lobes of the salivary gland and to the luminal side of the midgut epithelium, but not to the midgut surface facing the hemolymph or to ovaries. The coincidence of the tissues with which parasites and the SM1 peptide interact suggested that the parasite and peptide recognize the same surface ligand. In support of this hypothesis, the SM1 peptide strongly inhibited Plasmodium invasion of salivary gland and midgut epithelia. These experiments suggest a new strategy for the genetic manipulation of mosquito vectorial capacity. IS - 23 JF - Proc Natl Acad Sci U S A SN - 0027-8424 AD - Department of Genetics, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106-4955, USA. EP - 13281 TI - Targeting Plasmodium ligands on mosquito salivary glands and midgut with a phage display peptide library. VL - 98 SP - 13278 KW - anti_malarial_peptides KW - malaria KW - phage_display KW - plasmodium KW - screen KW - sm1 AU - Ghosh, AK AU - Ribolla, PE AU - Jacobs-Lorena, M PY - 2001/11/06/ UR - http://dx.doi.org/10.1073/pnas.241491198 ER -