Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems. Export

@article{citeulike:3282441, abstract = {Short interfering RNAs (siRNAs) are double-stranded RNAs of approximate to 21-25 nucleotides that have been shown to function as key intermediaries in triggering sequence-specific RNA degradation during posttranscriptional gene silencing in plants and RNA interference in invertebrates. siRNAs have a characteristic structure, with 5'-phosphate/3'-hydroxyl ends and a 2-base 3' overhang on each strand of the duplex. In this study, we present data that synthetic siRNAs can induce gene-specific inhibition of expression in Caenorhabditis elegans and in cell lines from humans and mice. In each case, the interference by siRNAs was superior to the inhibition of gene expression mediated by single-stranded antisense oligonucleotides. The siRNAs seem to avoid the well documented nonspecific effects triggered by longer double-stranded RNAs in mammalian cells. These observations may open a path toward the use of siRNAs as a reverse genetic and therapeutic tool in mammalian cells.}, author = {Caplen, N. J. and Parrish, S. and Imani, F. and Fire, A. and Morgan, R. A.}, citeulike-article-id = {3282441}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.171251798}, citeulike-linkout-1 = {http://www.pnas.org/content/98/17/9742.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/98/17/9742.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/11481446}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=11481446}, citeulike-linkout-5 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00004831}, doi = {10.1073/pnas.171251798}, journal = {Proceedings of the National Academy of Sciences USA}, keywords = {invertebrate, mechanism, rnai, vertebrate}, pages = {9742--9747}, posted-at = {2009-06-04 21:36:08}, priority = {5}, title = {Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems.}, url = {http://dx.doi.org/10.1073/pnas.171251798}, volume = {98}, year = {2001} }

TY - JOUR ID - citeulike:3282441 L3 - citeulike-article-id:3282441 N2 - Short interfering RNAs (siRNAs) are double-stranded RNAs of approximate to 21-25 nucleotides that have been shown to function as key intermediaries in triggering sequence-specific RNA degradation during posttranscriptional gene silencing in plants and RNA interference in invertebrates. siRNAs have a characteristic structure, with 5'-phosphate/3'-hydroxyl ends and a 2-base 3' overhang on each strand of the duplex. In this study, we present data that synthetic siRNAs can induce gene-specific inhibition of expression in Caenorhabditis elegans and in cell lines from humans and mice. In each case, the interference by siRNAs was superior to the inhibition of gene expression mediated by single-stranded antisense oligonucleotides. The siRNAs seem to avoid the well documented nonspecific effects triggered by longer double-stranded RNAs in mammalian cells. These observations may open a path toward the use of siRNAs as a reverse genetic and therapeutic tool in mammalian cells. JF - Proceedings of the National Academy of Sciences USA EP - 9747 TI - Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems. VL - 98 SP - 9742 KW - invertebrate KW - mechanism KW - rnai KW - vertebrate AU - Caplen, NJ AU - Parrish, S AU - Imani, F AU - Fire, A AU - Morgan, RA PY - 2001/// UR - http://dx.doi.org/10.1073/pnas.171251798 ER -