Dengue Virus Type 2 Infections of <italic>Aedes aegypti</italic> Are Modulated by the Mosquito's RNA Interference Pathway Export

@article{citeulike:4044642, abstract = {<title>Author Summary</title> <p>Dengue viruses, globally the most prevalent arboviruses, are transmitted to humans by persistently infected <italic>Aedes aegypti</italic> mosquitoes. Understanding the mechanisms mosquitoes use to modulate infections by these agents of serious human diseases should give us critical insights into virus–vector interactions leading to transmission. RNA interference (RNAi) is an innate defense mechanism used by invertebrates to inhibit RNA virus infections; however, little is known about the antiviral role of RNAi in mosquitoes. RNAi is triggered by double-stranded RNA, leading to degradation of RNA with sequence homology to the dsRNA trigger. We show that dengue virus type 2 (DENV2) infection of <italic>Ae. aegypti</italic> by the natural route generates dsRNA and DENV2-specific small interfering RNAs, hallmarks of the RNAi response; nevertheless, persistent infection of mosquitoes occurs, suggesting that DENV2 circumvents RNAi. We also show that DENV2 infection is modulated by RNAi, since impairment by silencing expression of genes encoding important sensor and effector proteins in the RNAi pathway increases virus replication in the vector and decreases the incubation period before virus transmission. Our findings indicate a significant role for RNAi in determining the mosquito vector's potential for transmitting human diseases.</p>}, author = {S\'{a}nchez-Vargas, Irma and Scott, Jaclyn C. and Poole-Smith, Katherine B. and Franz, Alexander W. and Barbosa-Solomieu, Val\'{e}rie and Wilusz, Jeffrey and Olson, Ken E. and Blair, Carol D.}, citeulike-article-id = {4044642}, citeulike-linkout-0 = {http://dx.doi.org/10.1371/journal.ppat.1000299}, day = {13}, doi = {10.1371/journal.ppat.1000299}, journal = {PLoS Pathog}, keywords = {aedes, antiviral, dengue, dengue\_review, mosquito, rnai}, month = {February}, number = {2}, pages = {e1000299+}, posted-at = {2009-02-16 18:39:53}, priority = {5}, publisher = {Public Library of Science}, title = {Dengue Virus Type 2 Infections of <italic>Aedes aegypti</italic> Are Modulated by the Mosquito's RNA Interference Pathway}, url = {http://dx.doi.org/10.1371/journal.ppat.1000299}, volume = {5}, year = {2009} }

TY - JOUR ID - citeulike:4044642 L3 - citeulike-article-id:4044642 N2 - <title>Author Summary</title> <p>Dengue viruses, globally the most prevalent arboviruses, are transmitted to humans by persistently infected <italic>Aedes aegypti</italic> mosquitoes. Understanding the mechanisms mosquitoes use to modulate infections by these agents of serious human diseases should give us critical insights into virus–vector interactions leading to transmission. RNA interference (RNAi) is an innate defense mechanism used by invertebrates to inhibit RNA virus infections; however, little is known about the antiviral role of RNAi in mosquitoes. RNAi is triggered by double-stranded RNA, leading to degradation of RNA with sequence homology to the dsRNA trigger. We show that dengue virus type 2 (DENV2) infection of <italic>Ae. aegypti</italic> by the natural route generates dsRNA and DENV2-specific small interfering RNAs, hallmarks of the RNAi response; nevertheless, persistent infection of mosquitoes occurs, suggesting that DENV2 circumvents RNAi. We also show that DENV2 infection is modulated by RNAi, since impairment by silencing expression of genes encoding important sensor and effector proteins in the RNAi pathway increases virus replication in the vector and decreases the incubation period before virus transmission. Our findings indicate a significant role for RNAi in determining the mosquito vector's potential for transmitting human diseases.</p> IS - 2 JF - PLoS Pathog TI - Dengue Virus Type 2 Infections of <italic>Aedes aegypti</italic> Are Modulated by the Mosquito's RNA Interference Pathway PB - Public Library of Science VL - 5 SP - e1000299 KW - aedes KW - antiviral KW - dengue KW - dengue_review KW - mosquito KW - rnai AU - Sánchez-Vargas, Irma AU - Scott, Jaclyn AU - Poole-Smith, Katherine AU - Franz, Alexander AU - Barbosa-Solomieu, Valérie AU - Wilusz, Jeffrey AU - Olson, Ken AU - Blair, Carol PY - 2009/02/13/ UR - http://dx.doi.org/10.1371/journal.ppat.1000299 ER -