Using bacteria to express and display anti-Plasmodium molecules in the mosquito midgut Export

@article{citeulike:4529370, abstract = {Bacteria capable of colonizing mosquito midguts are attractive vehicles for delivering anti-malaria molecules. We genetically engineered Escherichia coli to display two anti- Plasmodium effector molecules, SM1 and phospholipase-A(2), on their outer membrane. Both molecules significantly inhibited Plasmodium berghei development when engineered bacteria were fed to mosquitoes 24 h prior to an infective bloodmeal (SM1 = 41\%, PLA2 = 23\%). Furthermore, prevalence and numbers of engineered bacteria increased dramatically following a bloodmeal. However, E. coli survived poorly in mosquitoes. Therefore, Enterobacter agglomerans was isolated from mosquitoes and selected for midgut survival by multiple passages through mosquitoes. After four passages, E. agglomerans survivorship increased from 2 days to 2 weeks. Since E. agglomerans is non-pathogenic and widespread, it is an excellent candidate for paratransgenic control strategies.}, author = {Riehle, M. and Moreira, C. and Lampe, D. and Lauzon, C. and Jacobslorena, M.}, citeulike-article-id = {4529370}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.ijpara.2006.12.002}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0020751906004322}, doi = {10.1016/j.ijpara.2006.12.002}, issn = {00207519}, journal = {International Journal for Parasitology}, keywords = {anopheles, anti\_malarial\_peptides, bacteria, berghei, malaria, paratransgenesis, pla2, plasmodium, sm1}, month = {May}, number = {6}, pages = {595--603}, posted-at = {2009-05-16 21:46:07}, priority = {0}, title = {Using bacteria to express and display anti-Plasmodium molecules in the mosquito midgut}, url = {http://dx.doi.org/10.1016/j.ijpara.2006.12.002}, volume = {37}, year = {2007} }

TY - JOUR ID - citeulike:4529370 L3 - citeulike-article-id:4529370 N2 - Bacteria capable of colonizing mosquito midguts are attractive vehicles for delivering anti-malaria molecules. We genetically engineered Escherichia coli to display two anti- Plasmodium effector molecules, SM1 and phospholipase-A(2), on their outer membrane. Both molecules significantly inhibited Plasmodium berghei development when engineered bacteria were fed to mosquitoes 24 h prior to an infective bloodmeal (SM1 = 41%, PLA2 = 23%). Furthermore, prevalence and numbers of engineered bacteria increased dramatically following a bloodmeal. However, E. coli survived poorly in mosquitoes. Therefore, Enterobacter agglomerans was isolated from mosquitoes and selected for midgut survival by multiple passages through mosquitoes. After four passages, E. agglomerans survivorship increased from 2 days to 2 weeks. Since E. agglomerans is non-pathogenic and widespread, it is an excellent candidate for paratransgenic control strategies. IS - 6 JF - International Journal for Parasitology SN - 00207519 EP - 603 TI - Using bacteria to express and display anti-Plasmodium molecules in the mosquito midgut VL - 37 SP - 595 KW - anopheles KW - anti_malarial_peptides KW - bacteria KW - berghei KW - malaria KW - paratransgenesis KW - pla2 KW - plasmodium KW - sm1 AU - Riehle, M AU - Moreira, C AU - Lampe, D AU - Lauzon, C AU - Jacobslorena, M PY - 2007/05// UR - http://dx.doi.org/10.1016/j.ijpara.2006.12.002 ER -