Towards genetic manipulation of wild mosquito populations to combat malaria: advances and challenges Export

@article{citeulike:4545703, abstract = {Malaria kills millions of people every year, yet there has been little progress in controlling this disease. For transmission to occur, the malaria parasite has to complete a complex developmental cycle in the mosquito. The mosquito is therefore a potential weak link in malaria transmission, and generating mosquito populations that are refractory to the parasite is a potential means of controlling the disease. There has been considerable progress over the last decade towards developing the tools for creating a refractory mosquito. Accomplishments include germline transformation of several important mosquito vectors, the completed genomes of the mosquito Anopheles gambiae and the malaria parasite Plasmodium falciparum, and the identification of promoters and effector genes that confer resistance in the mosquito. These tools have provided researchers with the ability to engineer a refractory mosquito vector, but there are fundamental gaps in our knowledge of how to transfer this technology safely and effectively into field populations. This review considers strategies for interfering with Plasmodium development in the mosquito, together with issues related to the transfer of laboratory-acquired knowledge to the field, such as minimization of transgene fitness load to the mosquito, driving genes through populations, avoiding the selection of resistant strains, and how to produce and release populations of males only. 10.1242/jeb.00609}, author = {Riehle, Michael A. and Srinivasan, Prakash and Moreira, Cristina K. and Jacobs-Lorena, Marcelo}, citeulike-article-id = {4545703}, citeulike-linkout-0 = {http://dx.doi.org/10.1242/jeb.00609}, citeulike-linkout-1 = {http://jeb.biologists.org/cgi/content/abstract/206/21/3809?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=stephensi&searchid=1&FIRSTINDEX=10&resourcetype=HWFIG}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/14506216}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=14506216}, doi = {10.1242/jeb.00609}, journal = {J Exp Biol}, keywords = {anopheles, antibody, genetic\_drive, malaria, mosquito, pla2, plasmodium, resistance, review, sex\_specific\_lethality, sm1, transgenic, transmission\_blocking, vaccines}, month = {November}, number = {21}, pages = {3809--3816}, posted-at = {2009-05-19 18:20:34}, priority = {0}, title = {Towards genetic manipulation of wild mosquito populations to combat malaria: advances and challenges}, url = {http://dx.doi.org/10.1242/jeb.00609}, volume = {206}, year = {2003} }

TY - JOUR ID - citeulike:4545703 L3 - citeulike-article-id:4545703 N2 - Malaria kills millions of people every year, yet there has been little progress in controlling this disease. For transmission to occur, the malaria parasite has to complete a complex developmental cycle in the mosquito. The mosquito is therefore a potential weak link in malaria transmission, and generating mosquito populations that are refractory to the parasite is a potential means of controlling the disease. There has been considerable progress over the last decade towards developing the tools for creating a refractory mosquito. Accomplishments include germline transformation of several important mosquito vectors, the completed genomes of the mosquito Anopheles gambiae and the malaria parasite Plasmodium falciparum, and the identification of promoters and effector genes that confer resistance in the mosquito. These tools have provided researchers with the ability to engineer a refractory mosquito vector, but there are fundamental gaps in our knowledge of how to transfer this technology safely and effectively into field populations. This review considers strategies for interfering with Plasmodium development in the mosquito, together with issues related to the transfer of laboratory-acquired knowledge to the field, such as minimization of transgene fitness load to the mosquito, driving genes through populations, avoiding the selection of resistant strains, and how to produce and release populations of males only. 10.1242/jeb.00609 IS - 21 JF - J Exp Biol EP - 3816 TI - Towards genetic manipulation of wild mosquito populations to combat malaria: advances and challenges VL - 206 SP - 3809 KW - anopheles KW - antibody KW - genetic_drive KW - malaria KW - mosquito KW - pla2 KW - plasmodium KW - resistance KW - review KW - sex_specific_lethality KW - sm1 KW - transgenic KW - transmission_blocking KW - vaccines AU - Riehle, Michael AU - Srinivasan, Prakash AU - Moreira, Cristina AU - Jacobs-Lorena, Marcelo PY - 2003/11/01/ UR - http://dx.doi.org/10.1242/jeb.00609 ER -