Strongly Expanded 18S Ribosomal-Rna Genes Correlated with a Peculiar Morphology in the Insect Order of Strepsiptera Export

@article{Chalwatzis1994, abstract = {The 18S rRNA genes of the insects Xenos vesparum (Strepsiptera), its host Polistes dominulus (Insecta, Hymenoptera) and the beetle Melee proscarabaeus (Coleoptera) were cloned and sequenced. The Xenos gene was 3 316 bp long with a G+C content of 28.1\%. The low G+C content is caused by eight extremely A+T rich elements all intercalated into sites known to be variable in eukaryotic 18S rRNAs. Southern analysis showed that all 18S rRNA genes of Xenos contain A+T rich elements. 18S rRNA was reverse transcribed, the product amplified by the polymerase chain reaction and sequenced. This showed that the A+T rich elements were also presented in the mature rRNA. The 18S rRNA gene of another strepsipteran, Stylops melittae, was also shown to be longer than 3 kb and to contain A+T rich elements. The 18S rRNA genes of the host Polistes dominulus were 1 919 bp and those of Melee proscarabaeus 1 934 bp long with G+C contents of 49.3\% and 48.2\% respectively. Phylogenetic trees were constructed with the sequences of Xenos vesparum and six other insect species using maximum parsimony and the Fitch-Margoliash method. Both methods showed that Xenos groups together with Diptera and not with Coleoptera as suggested by a few morphological traits.}, address = {Th Darmstadt,Inst Zool,D-64287 Darmstadt,GermanyTh Darmstadt,Inst Mikrobiol,D-64287 Darmstadt,Germany}, author = {Chalwatzis, N. and Baur, A. and Stetzer, E. and Kinzelbach, R. and Zimmermann, F. K.}, citeulike-article-id = {1867626}, citeulike-linkout-0 = {#}, comment = {Qm179Times Cited:1Cited References Count:44}, journal = {Zoology-Analysis of Complex Systems}, keywords = {18s, bibtex-import, rdna}, pages = {115--126}, posted-at = {2007-11-05 13:41:50}, priority = {2}, title = {Strongly Expanded 18S Ribosomal-Rna Genes Correlated with a Peculiar Morphology in the Insect Order of Strepsiptera}, url = {<Go to ISI>://A1994QM17900005}, volume = {98}, year = {1994} }

TY - JOUR ID - Chalwatzis1994 L3 - citeulike-article-id:1867626 N2 - The 18S rRNA genes of the insects Xenos vesparum (Strepsiptera), its host Polistes dominulus (Insecta, Hymenoptera) and the beetle Melee proscarabaeus (Coleoptera) were cloned and sequenced. The Xenos gene was 3 316 bp long with a G+C content of 28.1\%. The low G+C content is caused by eight extremely A+T rich elements all intercalated into sites known to be variable in eukaryotic 18S rRNAs. Southern analysis showed that all 18S rRNA genes of Xenos contain A+T rich elements. 18S rRNA was reverse transcribed, the product amplified by the polymerase chain reaction and sequenced. This showed that the A+T rich elements were also presented in the mature rRNA. The 18S rRNA gene of another strepsipteran, Stylops melittae, was also shown to be longer than 3 kb and to contain A+T rich elements. The 18S rRNA genes of the host Polistes dominulus were 1 919 bp and those of Melee proscarabaeus 1 934 bp long with G+C contents of 49.3\% and 48.2\% respectively. Phylogenetic trees were constructed with the sequences of Xenos vesparum and six other insect species using maximum parsimony and the Fitch-Margoliash method. Both methods showed that Xenos groups together with Diptera and not with Coleoptera as suggested by a few morphological traits. JF - Zoology-Analysis of Complex Systems AD - Th Darmstadt,Inst Zool,D-64287 Darmstadt,GermanyTh Darmstadt,Inst Mikrobiol,D-64287 Darmstadt,Germany EP - 126 TI - Strongly Expanded 18S Ribosomal-Rna Genes Correlated with a Peculiar Morphology in the Insect Order of Strepsiptera VL - 98 SP - 115 KW - 18s KW - bibtex-import KW - rdna N1 - Qm179Times Cited:1Cited References Count:44 AU - Chalwatzis, N AU - Baur, A AU - Stetzer, E AU - Kinzelbach, R AU - Zimmermann, FK PY - 1994/// UR - # ER -