Destabilization of Tracts of Simple Repetitive DNA in Yeast by Mutations Affecting DNA Mismatch Repair Export

@article{Strand1993, abstract = {The genomes of all eukaryotes contain tracts of DNA in which a single base or a small number of bases is repeated. Expansions of such tracts have been associated with several human disorders including the fragile X syndrome1. In addition, simple repeats are unstable in certain forms of colorectal cancer, suggesting a defect in DNA replication or repair2-4. We show here that mutations in any three yeast genes involved in DNA mismatch repair (PMS1, MLH1 and MSH2) lead to 100- to 700-fold increases in tract instability, whereas mutations that eliminate the proof-reading function of DNA polymerases have little effect. The meiotic stability of the tracts is similar to the mitotic stability. Th results suggest that tract instability is associated with DNA polymerases slipping during replication, and that some types of colorectal cancer may reflect mutations in genes involved in DNA mismatch repair.}, address = {Strand, MUniv N Carolina,Dept Biol,Chapel Hill,Nc 27599Univ N Carolina,Curriculum Genet \& Molec Biol,Chapel Hill,Nc 27599Yale Univ,Sch Med,Dept Molec Biophys \& Biochem,New Haven,Ct 06510Yale Univ,Sch Med,Dept Therapeut Radiol,New Haven,Ct 06510}, author = {Strand, M. and Prolla, T. A. and Liskay, R. M. and Petes, T. D.}, citeulike-article-id = {1868305}, citeulike-linkout-0 = {#}, comment = {Lx471Times Cited:676Cited References Count:21}, journal = {Nature}, keywords = {5, activity, bibtex-import, exonuclease}, pages = {274--276}, posted-at = {2007-11-05 13:42:26}, priority = {2}, title = {Destabilization of Tracts of Simple Repetitive DNA in Yeast by Mutations Affecting DNA Mismatch Repair}, url = {<Go to ISI>://A1993LX47100061}, volume = {365}, year = {1993} }

TY - JOUR ID - Strand1993 L3 - citeulike-article-id:1868305 N2 - The genomes of all eukaryotes contain tracts of DNA in which a single base or a small number of bases is repeated. Expansions of such tracts have been associated with several human disorders including the fragile X syndrome1. In addition, simple repeats are unstable in certain forms of colorectal cancer, suggesting a defect in DNA replication or repair2-4. We show here that mutations in any three yeast genes involved in DNA mismatch repair (PMS1, MLH1 and MSH2) lead to 100- to 700-fold increases in tract instability, whereas mutations that eliminate the proof-reading function of DNA polymerases have little effect. The meiotic stability of the tracts is similar to the mitotic stability. Th results suggest that tract instability is associated with DNA polymerases slipping during replication, and that some types of colorectal cancer may reflect mutations in genes involved in DNA mismatch repair. JF - Nature AD - Strand, MUniv N Carolina,Dept Biol,Chapel Hill,Nc 27599Univ N Carolina,Curriculum Genet \& Molec Biol,Chapel Hill,Nc 27599Yale Univ,Sch Med,Dept Molec Biophys \& Biochem,New Haven,Ct 06510Yale Univ,Sch Med,Dept Therapeut Radiol,New Haven,Ct 06510 EP - 276 TI - Destabilization of Tracts of Simple Repetitive DNA in Yeast by Mutations Affecting DNA Mismatch Repair VL - 365 SP - 274 KW - 5 KW - activity KW - bibtex-import KW - exonuclease N1 - Lx471Times Cited:676Cited References Count:21 AU - Strand, M AU - Prolla, TA AU - Liskay, RM AU - Petes, TD PY - 1993/// UR - # ER -