Rapid DNA Library Construction for Functional Genomic and Metagenomic Screening Export

@article{citeulike:2428333, abstract = {A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins were cloned from bacteriophage genomes and an aerolysin was cloned from a metagenomic sample. 10.1128/AEM.01864-07}, author = {Schmitz, Jonathan E. and Daniel, Anu and Collin, Mattias and Schuch, Raymond and Fischetti, Vincent A.}, citeulike-article-id = {2428333}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/AEM.01864-07}, citeulike-linkout-1 = {http://aem.asm.org/cgi/content/abstract/74/5/1649}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18083885}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18083885}, day = {1}, doi = {10.1128/AEM.01864-07}, journal = {Appl. Environ. Microbiol.}, keywords = {aem}, month = {March}, number = {5}, pages = {1649--1652}, posted-at = {2008-02-26 03:57:41}, priority = {3}, title = {Rapid DNA Library Construction for Functional Genomic and Metagenomic Screening}, url = {http://dx.doi.org/10.1128/AEM.01864-07}, volume = {74}, year = {2008} }

TY - JOUR ID - citeulike:2428333 L3 - citeulike-article-id:2428333 N2 - A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins were cloned from bacteriophage genomes and an aerolysin was cloned from a metagenomic sample. 10.1128/AEM.01864-07 IS - 5 JF - Appl. Environ. Microbiol. EP - 1652 TI - Rapid DNA Library Construction for Functional Genomic and Metagenomic Screening VL - 74 SP - 1649 KW - aem AU - Schmitz, Jonathan AU - Daniel, Anu AU - Collin, Mattias AU - Schuch, Raymond AU - Fischetti, Vincent PY - 2008/03/01/ UR - http://dx.doi.org/10.1128/AEM.01864-07 ER -