Evidence that participate methane monooxygenase and ammonia monooxygenase may be evolutionarily related Export

@article{citeulike:2478254, abstract = {Abstract Genes encoding paniculate methane monooxygenase and ammonia monooxygenase share high sequence identity. Degenerate oligonucleotide primers were designed, based on regions of shared amino acid sequence between the 27-kDa polypeptides, which are believed to contain the active sites, of particulate methane monooxygenase and ammonia monooxygenase. A 525-bp internal DNA fragment of the genes encoding these polypeptides (pmoA and amoA) from a variety of methanotrophic and nitrifying bacteria was amplified by PCR, cloned and sequenced. Representatives of each of the phylogenetic groups of both methanotrophs (alpha- and gamma-Proteobacteria) and ammonia-oxidizing nitrifying bacteria (beta-and y-Proteobacteria) were included. Analysis of the predicted amino acid sequences of these genes revealed strong conservation of both primary and secondary structure. Nitrosococcus oceanus AmoA showed higher identity to PmoA sequences from other members of the gamma-Proteobacteria than to AmoA sequences. These results suggest that the particulate methane monooxygenase and ammonia monooxygenase are evolutionarily related enzymes despite their different physiological roles in these bacteria.}, author = {Holmes, Andrew J. and Costello, Andria and Lidstrom, Mary E. and Murrell, Colin J.}, citeulike-article-id = {2478254}, citeulike-linkout-0 = {http://www.blackwell-synergy.com/doi/abs/10.1111/j.1574-6968.1995.tb07834.x}, citeulike-linkout-1 = {http://dx.doi.org/10.1111/j.1574-6968.1995.tb07834.x}, doi = {10.1111/j.1574-6968.1995.tb07834.x}, journal = {FEMS Microbiology Letters}, keywords = {amo, fems, pmo}, number = {3}, pages = {203--208}, posted-at = {2008-03-06 10:51:15}, priority = {2}, title = {Evidence that participate methane monooxygenase and ammonia monooxygenase may be evolutionarily related}, url = {http://dx.doi.org/10.1111/j.1574-6968.1995.tb07834.x}, volume = {132}, year = {1995} }

TY - JOUR ID - citeulike:2478254 L3 - citeulike-article-id:2478254 N2 - Abstract Genes encoding paniculate methane monooxygenase and ammonia monooxygenase share high sequence identity. Degenerate oligonucleotide primers were designed, based on regions of shared amino acid sequence between the 27-kDa polypeptides, which are believed to contain the active sites, of particulate methane monooxygenase and ammonia monooxygenase. A 525-bp internal DNA fragment of the genes encoding these polypeptides (pmoA and amoA) from a variety of methanotrophic and nitrifying bacteria was amplified by PCR, cloned and sequenced. Representatives of each of the phylogenetic groups of both methanotrophs (alpha- and gamma-Proteobacteria) and ammonia-oxidizing nitrifying bacteria (beta-and y-Proteobacteria) were included. Analysis of the predicted amino acid sequences of these genes revealed strong conservation of both primary and secondary structure. Nitrosococcus oceanus AmoA showed higher identity to PmoA sequences from other members of the gamma-Proteobacteria than to AmoA sequences. These results suggest that the particulate methane monooxygenase and ammonia monooxygenase are evolutionarily related enzymes despite their different physiological roles in these bacteria. IS - 3 JF - FEMS Microbiology Letters EP - 208 TI - Evidence that participate methane monooxygenase and ammonia monooxygenase may be evolutionarily related VL - 132 SP - 203 KW - amo KW - fems KW - pmo AU - Holmes, Andrew AU - Costello, Andria AU - Lidstrom, Mary AU - Murrell, Colin PY - 1995/// UR - http://dx.doi.org/10.1111/j.1574-6968.1995.tb07834.x ER -