Cell proliferation at 122°C and isotopically heavy CH4 production by a hyperthermophilic methanogen under high-pressure cultivation Export

@article{citeulike:4185319, abstract = {10.1073/pnas.0712334105 We have developed a technique for cultivation of chemolithoautotrophs under high hydrostatic pressures that is successfully applicable to various types of deep-sea chemolithoautotrophs, including methanogens. It is based on a glass-syringe-sealing liquid medium and gas mixture used in conjunction with a butyl rubber piston and a metallic needle stuck into butyl rubber. By using this technique, growth, survival, and methane production of a newly isolated, hyperthermophilic methanogen strain 116 are characterized under high temperatures and hydrostatic pressures. Elevated hydrostatic pressures extend the temperature maximum for possible cell proliferation from 116°C at 0.4 MPa to 122°C at 20 MPa, providing the potential for growth even at 122°C under an high pressure. In addition, piezophilic growth significantly affected stable carbon isotope fractionation of methanogenesis from CO. Under conventional growth conditions, the isotope fractionation of methanogenesis by strain 116 was similar to values (−34‰ to−27‰) previously reported for other hydrogenotrophic methanogens. However, under high hydrostatic pressures, the isotope fractionation effect became much smaller (<−12‰), and the kinetic isotope effect at 122°C and 40 MPa was −9.4‰, which is one of the smallest effects ever reported. This observation will shed light on the sources and production mechanisms of deep-sea methane.}, author = {Takai, Ken and Nakamura, Kentaro and Toki, Tomohiro and Tsunogai, Urumu and Miyazaki, Masayuki and Miyazaki, Junichi and Hirayama, Hisako and Nakagawa, Satoshi and Nunoura, Takuro and Horikoshi, Koki}, citeulike-article-id = {4185319}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0712334105}, citeulike-linkout-1 = {http://www.pnas.org/content/105/31/10949.long.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/105/31/10949.long.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/18664583}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=18664583}, day = {5}, doi = {10.1073/pnas.0712334105}, journal = {Proceedings of the National Academy of Sciences}, keywords = {archaea, hydrogen, hyperthermophile, water-rock}, month = {August}, number = {31}, pages = {10949--10954}, posted-at = {2009-03-17 06:37:27}, priority = {2}, title = {Cell proliferation at 122°C and isotopically heavy CH4 production by a hyperthermophilic methanogen under high-pressure cultivation}, url = {http://dx.doi.org/10.1073/pnas.0712334105}, volume = {105}, year = {2008} }

TY - JOUR ID - citeulike:4185319 L3 - citeulike-article-id:4185319 N2 - 10.1073/pnas.0712334105 We have developed a technique for cultivation of chemolithoautotrophs under high hydrostatic pressures that is successfully applicable to various types of deep-sea chemolithoautotrophs, including methanogens. It is based on a glass-syringe-sealing liquid medium and gas mixture used in conjunction with a butyl rubber piston and a metallic needle stuck into butyl rubber. By using this technique, growth, survival, and methane production of a newly isolated, hyperthermophilic methanogen strain 116 are characterized under high temperatures and hydrostatic pressures. Elevated hydrostatic pressures extend the temperature maximum for possible cell proliferation from 116°C at 0.4 MPa to 122°C at 20 MPa, providing the potential for growth even at 122°C under an high pressure. In addition, piezophilic growth significantly affected stable carbon isotope fractionation of methanogenesis from CO. Under conventional growth conditions, the isotope fractionation of methanogenesis by strain 116 was similar to values (−34‰ to−27‰) previously reported for other hydrogenotrophic methanogens. However, under high hydrostatic pressures, the isotope fractionation effect became much smaller (<−12‰), and the kinetic isotope effect at 122°C and 40 MPa was −9.4‰, which is one of the smallest effects ever reported. This observation will shed light on the sources and production mechanisms of deep-sea methane. IS - 31 JF - Proceedings of the National Academy of Sciences EP - 10954 TI - Cell proliferation at 122°C and isotopically heavy CH4 production by a hyperthermophilic methanogen under high-pressure cultivation VL - 105 SP - 10949 KW - archaea KW - hydrogen KW - hyperthermophile KW - water-rock AU - Takai, Ken AU - Nakamura, Kentaro AU - Toki, Tomohiro AU - Tsunogai, Urumu AU - Miyazaki, Masayuki AU - Miyazaki, Junichi AU - Hirayama, Hisako AU - Nakagawa, Satoshi AU - Nunoura, Takuro AU - Horikoshi, Koki PY - 2008/08/05/ UR - http://dx.doi.org/10.1073/pnas.0712334105 ER -