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Cultivation of Methanogens under Low-Hydrogen Conditions by Using the Coculture Method Export

by: Sanae Sakai, Hiroyuki Imachi, Yuji Sekiguchi, I-Cheng Tseng, Akiyoshi Ohashi, Hideki Harada, Yoichi Kamagata
Appl. Environ. Microbiol., Vol. 75, No. 14. (15 July 2009), pp. 4892-4896.

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cultivation hydrogen methanogen

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We previously reported the isolation of novel methanogens by using a new cultivation method, referred to as the coculture method. Here, we extended our coculture method to various anaerobic environmental samples. As a result, we successfully cultivated some uncharacterized methanogens in coculture enrichments and eventually isolated a new methanogen, within the order Methanomicrobiales. 10.1128/AEM.02835-08


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