Stretching of Homopolymeric RNA Reveals Single-Stranded Helices and Base-Stacking

by: Yeonee Seol, Gary M Skinner, Koen Visscher, Arnaud Buhot, Avraham Halperin

Physical Review Letters, Vol. 98, No. 15. (2007)

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Abstract

We have found strong supporting evidence for the helical structures of single-stranded nucleic acids by stretching individual molecules of polyadenylic acid [poly(A)] and polycytidylic acid [poly(C)]. Analyzing the force versus extension data using a two-state elastic model in which random-coil domains alternate with rigid helical domains allows one to extract the thermodynamic and structural properties. In addition, it also yields moderate to low cooperativity of the helix-coil transition for poly(A) and poly(C), respectively.

@article{citeulike:1399023, abstract = {We have found strong supporting evidence for the helical structures of single-stranded nucleic acids by stretching individual molecules of polyadenylic acid [poly(A)] and polycytidylic acid [poly(C)]. Analyzing the force versus extension data using a two-state elastic model in which random-coil domains alternate with rigid helical domains allows one to extract the thermodynamic and structural properties. In addition, it also yields moderate to low cooperativity of the helix-coil transition for poly(A) and poly(C), respectively.}, author = {Seol, Yeonee and Skinner, Gary M. and Visscher, Koen and Buhot, Arnaud and Halperin, Avraham }, citeulike-article-id = {1399023}, doi = {http://dx.doi.org/10.1103/PhysRevLett.98.158103}, journal = {Physical Review Letters}, keywords = {afm, biophysics, force, rna, single-molecule, spectroscopy}, number = {15}, posted-at = {2007-06-19 18:45:56}, priority = {4}, publisher = {APS}, title = {Stretching of Homopolymeric RNA Reveals Single-Stranded Helices and Base-Stacking}, url = {http://dx.doi.org/10.1103/PhysRevLett.98.158103}, volume = {98}, year = {2007} }

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TY - JOUR ID - citeulike:1399023 L3 - citeulike-article-id:1399023 TI - Stretching of Homopolymeric RNA Reveals Single-Stranded Helices and Base-Stacking JF - Physical Review Letters VL - 98 IS - 15 PB - APS N2 - We have found strong supporting evidence for the helical structures of single-stranded nucleic acids by stretching individual molecules of polyadenylic acid [poly(A)] and polycytidylic acid [poly(C)]. Analyzing the force versus extension data using a two-state elastic model in which random-coil domains alternate with rigid helical domains allows one to extract the thermodynamic and structural properties. In addition, it also yields moderate to low cooperativity of the helix-coil transition for poly(A) and poly(C), respectively. KW - afm KW - biophysics KW - force KW - rna KW - single-molecule KW - spectroscopy AU - Seol, Yeonee AU - Skinner, Gary AU - Visscher, Koen AU - Buhot, Arnaud AU - Halperin, Avraham PY - 2007/// UR - http://dx.doi.org/10.1103/PhysRevLett.98.158103 ER -

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