Cardiac progenitor cells from adult myocardium: Homing, differentiation, and fusion after infarction

@article{citeulike:2835646, abstract = {Potential repair by cell grafting or mobilizing endogenous cells holds particular attraction in heart disease, where the meager capacity for cardiomyocyte proliferation likely contributes to the irreversibility of heart failure. Whether cardiac progenitors exist in adult myocardium itself is unanswered, as is the question whether undifferentiated cardiac precursor cells merely fuse with preexisting myocytes. Here we report the existence of adult heart-derived cardiac progenitor cells expressing stem cell antigen-1. Initially, the cells express neither cardiac structural genes nor Nkx2.5 but differentiate in vitro in response to 5'-azacytidine, in part depending on Bmpr1a, a receptor for bone morphogenetic proteins. Given intravenously after ischemia/reperfusion, cardiac stem cell antigen 1 cells home to injured myocardium. By using a Cre/Lox donor/recipient pair ({alpha}MHC-Cre/R26R), differentiation was shown to occur roughly equally, with and without fusion to host cells. 10.1073/pnas.2132126100}, author = {Oh, Hidemasa and Bradfute, Steven B. and Gallardo, Teresa D. and Nakamura, Teruya and Gaussin, Vinciane and Mishina, Yuji and Pocius, Jennifer and Michael, Lloyd H. and Behringer, Richard R. and Garry, Daniel J. and Entman, Mark L. and Schneider, Michael D. }, citeulike-article-id = {2835646}, doi = {http://dx.doi.org/10.1073/pnas.2132126100}, journal = {Proceedings of the National Academy of Sciences}, month = {October}, number = {21}, pages = {12313--12318}, posted-at = {2008-05-26 21:29:36}, priority = {4}, title = {Cardiac progenitor cells from adult myocardium: Homing, differentiation, and fusion after infarction}, url = {http://dx.doi.org/10.1073/pnas.2132126100}, volume = {100}, year = {2003} }

TY - JOUR ID - citeulike:2835646 L3 - citeulike-article-id:2835646 TI - Cardiac progenitor cells from adult myocardium: Homing, differentiation, and fusion after infarction JF - Proceedings of the National Academy of Sciences VL - 100 IS - 21 SP - 12313 EP - 12318 N2 - Potential repair by cell grafting or mobilizing endogenous cells holds particular attraction in heart disease, where the meager capacity for cardiomyocyte proliferation likely contributes to the irreversibility of heart failure. Whether cardiac progenitors exist in adult myocardium itself is unanswered, as is the question whether undifferentiated cardiac precursor cells merely fuse with preexisting myocytes. Here we report the existence of adult heart-derived cardiac progenitor cells expressing stem cell antigen-1. Initially, the cells express neither cardiac structural genes nor Nkx2.5 but differentiate in vitro in response to 5'-azacytidine, in part depending on Bmpr1a, a receptor for bone morphogenetic proteins. Given intravenously after ischemia/reperfusion, cardiac stem cell antigen 1 cells home to injured myocardium. By using a Cre/Lox donor/recipient pair ({alpha}MHC-Cre/R26R), differentiation was shown to occur roughly equally, with and without fusion to host cells. 10.1073/pnas.2132126100 AU - Oh, Hidemasa AU - Bradfute, Steven AU - Gallardo, Teresa AU - Nakamura, Teruya AU - Gaussin, Vinciane AU - Mishina, Yuji AU - Pocius, Jennifer AU - Michael, Lloyd AU - Behringer, Richard AU - Garry, Daniel AU - Entman, Mark AU - Schneider, Michael PY - 2003/10/14/ UR - http://dx.doi.org/10.1073/pnas.2132126100 ER -