Virus Attenuation by Genome-Scale Changes in Codon Pair Bias Export

@article{citeulike:2937482, abstract = {As a result of the redundancy of the genetic code, adjacent pairs of amino acids can be encoded by as many as 36 different pairs of synonymous codons. A species-specific "codon pair bias" provides that some synonymous codon pairs are used more or less frequently than statistically predicted. We synthesized de novo large DNA molecules using hundreds of over-or underrepresented synonymous codon pairs to encode the poliovirus capsid protein. Underrepresented codon pairs caused decreased rates of protein translation, and polioviruses containing such amino acid-independent changes were attenuated in mice. Polioviruses thus customized were used to immunize mice and provided protective immunity after challenge. This "death by a thousand cuts" strategy could be generally applicable to attenuating many kinds of viruses. 10.1126/science.1155761}, author = {Coleman, Robert J. and Papamichail, Dimitris and Skiena, Steven and Futcher, Bruce and Wimmer, Eckard and Mueller, Steffen}, citeulike-article-id = {2937482}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1155761}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/320/5884/1784}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18583614}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18583614}, day = {27}, doi = {10.1126/science.1155761}, journal = {Science}, keywords = {bias, codon, evolution, viral}, month = {June}, number = {5884}, pages = {1784--1787}, posted-at = {2008-06-27 22:22:19}, priority = {2}, title = {Virus Attenuation by Genome-Scale Changes in Codon Pair Bias}, url = {http://dx.doi.org/10.1126/science.1155761}, volume = {320}, year = {2008} }

TY - JOUR ID - citeulike:2937482 L3 - citeulike-article-id:2937482 N2 - As a result of the redundancy of the genetic code, adjacent pairs of amino acids can be encoded by as many as 36 different pairs of synonymous codons. A species-specific "codon pair bias" provides that some synonymous codon pairs are used more or less frequently than statistically predicted. We synthesized de novo large DNA molecules using hundreds of over-or underrepresented synonymous codon pairs to encode the poliovirus capsid protein. Underrepresented codon pairs caused decreased rates of protein translation, and polioviruses containing such amino acid-independent changes were attenuated in mice. Polioviruses thus customized were used to immunize mice and provided protective immunity after challenge. This "death by a thousand cuts" strategy could be generally applicable to attenuating many kinds of viruses. 10.1126/science.1155761 IS - 5884 JF - Science EP - 1787 TI - Virus Attenuation by Genome-Scale Changes in Codon Pair Bias VL - 320 SP - 1784 KW - bias KW - codon KW - evolution KW - viral AU - Coleman, Robert AU - Papamichail, Dimitris AU - Skiena, Steven AU - Futcher, Bruce AU - Wimmer, Eckard AU - Mueller, Steffen PY - 2008/06/27/ UR - http://dx.doi.org/10.1126/science.1155761 ER -