Force-induced bidirectional stepping of cytoplasmic Dynein. Export

@article{GennerichA_ForceIndBidSteppngDynein_Cell_2007, abstract = {Cytoplasmic dynein is a minus-end-directed microtubule motor whose mechanism of movement remains poorly understood. Here, we use optical tweezers to examine the force-dependent stepping behavior of yeast cytoplasmic dynein. We find that dynein primarily advances in 8 nm increments but takes other sized steps (4-24 nm) as well. An opposing force induces more frequent backward stepping by dynein, and the motor walks backward toward the microtubule plus end at loads above its stall force of 7 pN. Remarkably, in the absence of ATP, dynein steps processively along microtubules under an external load, with less force required for minus-end- than for plus-end-directed movement. This nucleotide-independent walking reveals that force alone can drive repetitive microtubule detachment-attachment cycles of dynein's motor domains. These results suggest a model for how dynein's two motor domains coordinate their activities during normal processive motility and provide new clues for understanding dynein-based motility in living cells.}, address = {The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA 94158-2200, USA.}, author = {Gennerich, A. and Carter, A. P. and Reck-Peterson, S. L. and Vale, R. D.}, citeulike-article-id = {2162970}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.cell.2007.10.016}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18045537}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18045537}, citeulike-linkout-3 = {http://www.sciencedirect.com/science/article/B6WSN-4R7M4V3-J/2/95cc9525ba2cef224e6655ce9b8f19be}, day = {30}, doi = {10.1016/j.cell.2007.10.016}, issn = {0092-8674}, journal = {Cell}, keywords = {bidirectional, dynein, force, stepping, vale}, month = {November}, number = {5}, pages = {952--965}, posted-at = {2008-05-26 11:44:30}, priority = {0}, title = {Force-induced bidirectional stepping of cytoplasmic Dynein.}, url = {http://dx.doi.org/10.1016/j.cell.2007.10.016}, volume = {131}, year = {2007} }

TY - JOUR ID - GennerichA_ForceIndBidSteppngDynein_Cell_2007 L3 - citeulike-article-id:2162970 N2 - Cytoplasmic dynein is a minus-end-directed microtubule motor whose mechanism of movement remains poorly understood. Here, we use optical tweezers to examine the force-dependent stepping behavior of yeast cytoplasmic dynein. We find that dynein primarily advances in 8 nm increments but takes other sized steps (4-24 nm) as well. An opposing force induces more frequent backward stepping by dynein, and the motor walks backward toward the microtubule plus end at loads above its stall force of 7 pN. Remarkably, in the absence of ATP, dynein steps processively along microtubules under an external load, with less force required for minus-end- than for plus-end-directed movement. This nucleotide-independent walking reveals that force alone can drive repetitive microtubule detachment-attachment cycles of dynein's motor domains. These results suggest a model for how dynein's two motor domains coordinate their activities during normal processive motility and provide new clues for understanding dynein-based motility in living cells. IS - 5 JF - Cell SN - 0092-8674 AD - The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA 94158-2200, USA. EP - 965 TI - Force-induced bidirectional stepping of cytoplasmic Dynein. VL - 131 SP - 952 KW - bidirectional KW - dynein KW - force KW - stepping KW - vale AU - Gennerich, A AU - Carter, AP AU - Reck-Peterson, SL AU - Vale, RD PY - 2007/11/30/ UR - http://dx.doi.org/10.1016/j.cell.2007.10.016 ER -