Combined intracellular three-dimensional imaging and selective nanosurgery by a nonlinear microscope. Export

@article{SacconiL_3D_ImagNanosurgery2Photon_JBO_2005, abstract = {We use near-IR femtosecond laser pulses for a combination of microscopy and nanosurgery on fluorescently labeled structures within living cells. Three-dimensional reconstructions of microtubule structures tagged with green fluorescent protein (GFP) are made during different phases of the cell cycle. Further, the microtubules are dissected using the same laser beam but with a higher laser power than for microscopy. We establish the viability of this technique for the cells of a fission yeast, which is a common model to study the mechanics of cell division. We show that nanosurgery can be performed with submicrometer precision and without visible collateral damage to the cell. The energy is primarily absorbed by the GFP molecules, and not by other native structures in the cell. GFP is particularly suitable for multiphoton excitation, as its excitation wavelength near 900 nm is benign for most cellular structures. The ability to use GFP to label structures for destruction by multiphoton excitation may be a valuable tool in cell biology.}, address = {University of Trento, via Sommarive 14, 38050 Povo (TN), Italy.}, author = {Sacconi, L. and Tolic-Norrelykke, I. M. and Antolini, R. and Pavone, F. S.}, citeulike-article-id = {2641900}, citeulike-linkout-0 = {http://dx.doi.org/10.1117/1.1854675}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15847583}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15847583}, doi = {10.1117/1.1854675}, issn = {1083-3668}, journal = {Journal of biomedical optics}, keywords = {2-photon, ablation, laser, microscope, nanosurgery}, number = {1}, posted-at = {2008-11-06 10:34:54}, priority = {0}, title = {Combined intracellular three-dimensional imaging and selective nanosurgery by a nonlinear microscope.}, url = {http://dx.doi.org/10.1117/1.1854675}, volume = {10}, year = {2005} }

TY - JOUR ID - SacconiL_3D_ImagNanosurgery2Photon_JBO_2005 L3 - citeulike-article-id:2641900 N2 - We use near-IR femtosecond laser pulses for a combination of microscopy and nanosurgery on fluorescently labeled structures within living cells. Three-dimensional reconstructions of microtubule structures tagged with green fluorescent protein (GFP) are made during different phases of the cell cycle. Further, the microtubules are dissected using the same laser beam but with a higher laser power than for microscopy. We establish the viability of this technique for the cells of a fission yeast, which is a common model to study the mechanics of cell division. We show that nanosurgery can be performed with submicrometer precision and without visible collateral damage to the cell. The energy is primarily absorbed by the GFP molecules, and not by other native structures in the cell. GFP is particularly suitable for multiphoton excitation, as its excitation wavelength near 900 nm is benign for most cellular structures. The ability to use GFP to label structures for destruction by multiphoton excitation may be a valuable tool in cell biology. IS - 1 JF - Journal of biomedical optics SN - 1083-3668 AD - University of Trento, via Sommarive 14, 38050 Povo (TN), Italy. TI - Combined intracellular three-dimensional imaging and selective nanosurgery by a nonlinear microscope. VL - 10 KW - 2-photon KW - ablation KW - laser KW - microscope KW - nanosurgery AU - Sacconi, L AU - Tolic-Norrelykke, IM AU - Antolini, R AU - Pavone, FS PY - 2005///b UR - http://dx.doi.org/10.1117/1.1854675 ER -