The cortical protein Num1p is essential for dynein-dependent interactions of microtubules with the cortex. Export

@article{Heil-ChapdelaineRA_Num1_BuddingYeast_JCB_2000, abstract = {In budding yeast, the mitotic spindle moves into the neck between the mother and bud via dynein-dependent sliding of cytoplasmic microtubules along the cortex of the bud. How dynein and microtubules interact with the cortex is unknown. We found that cells lacking Num1p failed to exhibit dynein-dependent microtubule sliding in the bud, resulting in defective mitotic spindle movement and nuclear segregation. Num1p localized to the bud cortex, and that localization was independent of microtubules, dynein, or dynactin. These data are consistent with Num1p being an essential element of the cortical attachment mechanism for dynein-dependent sliding of microtubules in the bud.}, author = {Heil-Chapdelaine, R. A. and Oberle, J. R. and Cooper, J. A.}, citeulike-article-id = {3466073}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/11121446}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=11121446}, day = {11}, issn = {0021-9525}, journal = {The Journal of cell biology}, keywords = {num1, yeast}, month = {December}, number = {6}, pages = {1337--1344}, posted-at = {2008-10-30 16:44:56}, priority = {0}, title = {The cortical protein Num1p is essential for dynein-dependent interactions of microtubules with the cortex.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/11121446}, volume = {151}, year = {2000} }

TY - JOUR ID - Heil-ChapdelaineRA_Num1_BuddingYeast_JCB_2000 L3 - citeulike-article-id:3466073 N2 - In budding yeast, the mitotic spindle moves into the neck between the mother and bud via dynein-dependent sliding of cytoplasmic microtubules along the cortex of the bud. How dynein and microtubules interact with the cortex is unknown. We found that cells lacking Num1p failed to exhibit dynein-dependent microtubule sliding in the bud, resulting in defective mitotic spindle movement and nuclear segregation. Num1p localized to the bud cortex, and that localization was independent of microtubules, dynein, or dynactin. These data are consistent with Num1p being an essential element of the cortical attachment mechanism for dynein-dependent sliding of microtubules in the bud. IS - 6 JF - The Journal of cell biology SN - 0021-9525 EP - 1344 TI - The cortical protein Num1p is essential for dynein-dependent interactions of microtubules with the cortex. VL - 151 SP - 1337 KW - num1 KW - yeast AU - Heil-Chapdelaine, RA AU - Oberle, JR AU - Cooper, JA PY - 2000/12/11/ UR - http://view.ncbi.nlm.nih.gov/pubmed/11121446 ER -