Analysis of the Role of Astral Rays in Pronuclear Migration in Sand Dollar Eggs by the Colcemid-UV Method Export

@article{HamaguchiMS&HiramotoY_AstralMT_raysPronuclMigr_DevGrowthDiffer_1986, abstract = {The formation and migration of the sperm aster, and the migration of male and female pronuclei during fertilization were investigated in the eggs of the sand dollar, Clypeaster japonicus using the Colcemid-UV method. When an egg in Colcemid sea water was irradiated locally with UV light (about 365 nm wavelength) at a limited region containing sperm head, a sperm aster formed in this region, and migrated to the center of the UV-irradiated region during its formation. When the UV-irradiated region was displaced or its shape was changed after the formation of the sperm aster, the aster migrated to the center of the new UV-irradiated region. The direction of the migration of the sperm aster coincided with the direction of the longest astral rays. Direct contact between astral rays and the egg surface was not essential for sperm aster migration. When a region containing both the sperm centrosome and the female pronucleus was irradiated with UV light, the female pronucleus migrated toward the center of the sperm aster after they were connected by astral rays. The migration was suppressed when UV light was shaded over the region between the aster and the female pronucleus. These results suggest that the female pronucleus migrates to the sperm aster by attractive force between them.}, address = {Biological Laboratory, Tokyo Institute of Technology, O-okayama, Meguro-ku, Tokyo 152; Department of Cell Biology, National Institute for Basic Biology, Okazaki 444, Japan}, author = {Hamaguchi, Miyako S. and Hiramoto, Yukio}, citeulike-article-id = {3484881}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1440-169X.1986.00143.x}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/120702953/ABSTRACT}, doi = {10.1111/j.1440-169X.1986.00143.x}, journal = {Development Growth \& Differentiation}, keywords = {centrosome, dollar, eggs, hamaguchi, hiramoto, microtubule, sand}, number = {2}, pages = {143--156}, posted-at = {2008-11-06 15:44:42}, priority = {0}, title = {Analysis of the Role of Astral Rays in Pronuclear Migration in Sand Dollar Eggs by the Colcemid-UV Method}, url = {http://dx.doi.org/10.1111/j.1440-169X.1986.00143.x}, volume = {28}, year = {1986} }

TY - JOUR ID - HamaguchiMS&HiramotoY_AstralMT_raysPronuclMigr_DevGrowthDiffer_1986 L3 - citeulike-article-id:3484881 N2 - The formation and migration of the sperm aster, and the migration of male and female pronuclei during fertilization were investigated in the eggs of the sand dollar, Clypeaster japonicus using the Colcemid-UV method. When an egg in Colcemid sea water was irradiated locally with UV light (about 365 nm wavelength) at a limited region containing sperm head, a sperm aster formed in this region, and migrated to the center of the UV-irradiated region during its formation. When the UV-irradiated region was displaced or its shape was changed after the formation of the sperm aster, the aster migrated to the center of the new UV-irradiated region. The direction of the migration of the sperm aster coincided with the direction of the longest astral rays. Direct contact between astral rays and the egg surface was not essential for sperm aster migration. When a region containing both the sperm centrosome and the female pronucleus was irradiated with UV light, the female pronucleus migrated toward the center of the sperm aster after they were connected by astral rays. The migration was suppressed when UV light was shaded over the region between the aster and the female pronucleus. These results suggest that the female pronucleus migrates to the sperm aster by attractive force between them. IS - 2 JF - Development Growth & Differentiation AD - Biological Laboratory, Tokyo Institute of Technology, O-okayama, Meguro-ku, Tokyo 152; Department of Cell Biology, National Institute for Basic Biology, Okazaki 444, Japan EP - 156 TI - Analysis of the Role of Astral Rays in Pronuclear Migration in Sand Dollar Eggs by the Colcemid-UV Method VL - 28 SP - 143 KW - centrosome KW - dollar KW - eggs KW - hamaguchi KW - hiramoto KW - microtubule KW - sand AU - Hamaguchi, Miyako AU - Hiramoto, Yukio PY - 1986/// UR - http://dx.doi.org/10.1111/j.1440-169X.1986.00143.x ER -