RNAi in Budding Yeast Export

@article{citeulike:5781514, abstract = {RNA interference (RNAi), a gene-silencing pathway triggered by double-stranded RNA, is conserved in diverse eukaryotic species but has been lost in the model budding yeast Saccharomyces cerevisiae. Here, we show that RNAi is present in other budding yeast species, including Saccharomyces castellii and Candida albicans. These species use noncanonical Dicer proteins to generate small interfering RNAs, which mostly correspond to transposable elements and Y' subtelomeric repeats. In S. castellii, RNAi mutants are viable but have excess Y' messenger RNA levels. In S. cerevisiae, introducing Dicer and Argonaute of S. castellii restores RNAi, and the reconstituted pathway silences endogenous retrotransposons. These results identify a previously unknown class of Dicer proteins, bring the tool of RNAi to the study of budding yeasts, and bring the tools of budding yeast to the study of RNAi. 10.1126/science.1176945}, author = {Drinnenberg, Ines A. and Weinberg, David E. and Xie, Kathleen T. and Mower, Jeffrey P. and Wolfe, Kenneth H. and Fink, Gerald R. and Bartel, David P.}, citeulike-article-id = {5781514}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1176945}, citeulike-linkout-1 = {http://www.sciencemag.org/content/1176945/.abstract}, citeulike-linkout-2 = {http://www.sciencemag.org/content/1176945/.full.pdf}, citeulike-linkout-3 = {http://www.sciencemag.org/cgi/content/abstract/326/5952/544}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/19745116}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=19745116}, day = {23}, doi = {10.1126/science.1176945}, issn = {1095-9203}, journal = {Science}, keywords = {genomics, rna\_toolkit, rnai, yeast\_saccharomyces}, month = {October}, number = {5952}, pages = {544--550}, posted-at = {2009-11-09 14:10:28}, priority = {3}, title = {RNAi in Budding Yeast}, url = {http://dx.doi.org/10.1126/science.1176945}, volume = {326}, year = {2009} }

TY - JOUR ID - citeulike:5781514 L3 - citeulike-article-id:5781514 N2 - RNA interference (RNAi), a gene-silencing pathway triggered by double-stranded RNA, is conserved in diverse eukaryotic species but has been lost in the model budding yeast Saccharomyces cerevisiae. Here, we show that RNAi is present in other budding yeast species, including Saccharomyces castellii and Candida albicans. These species use noncanonical Dicer proteins to generate small interfering RNAs, which mostly correspond to transposable elements and Y' subtelomeric repeats. In S. castellii, RNAi mutants are viable but have excess Y' messenger RNA levels. In S. cerevisiae, introducing Dicer and Argonaute of S. castellii restores RNAi, and the reconstituted pathway silences endogenous retrotransposons. These results identify a previously unknown class of Dicer proteins, bring the tool of RNAi to the study of budding yeasts, and bring the tools of budding yeast to the study of RNAi. 10.1126/science.1176945 IS - 5952 JF - Science SN - 1095-9203 EP - 550 TI - RNAi in Budding Yeast VL - 326 SP - 544 KW - genomics KW - rna_toolkit KW - rnai KW - yeast_saccharomyces AU - Drinnenberg, Ines AU - Weinberg, David AU - Xie, Kathleen AU - Mower, Jeffrey AU - Wolfe, Kenneth AU - Fink, Gerald AU - Bartel, David PY - 2009/10/23/ UR - http://dx.doi.org/10.1126/science.1176945 ER -