In Vivo Molecular Imaging of Stretch-Induced Tissue Factor in Carotid Arteries with Ligand-Targeted Nanoparticles

@article{citeulike:2460779, abstract = {Molecular imaging permits tissues to be functionally characterized by identification of specific cell-surface receptors with targeted contrast agents. In our study, a ligand-targeted acoustic nanoparticle system was used to identify the angioplasty-induced expression of tissue factor by smooth muscle cells within the tunica media. Pig carotid arteries were overstretched bilaterally with balloon catheters, treated with a tissue factor-targeted or a control nanoparticle system, and imaged with intravascular ultrasound (20 MHz) before and after treatment. Carotid wall acoustic reflectivities were unaffected by overstretch injury. Tissue factor-targeted nanoemulsion bound and increased the echogenicity of smooth muscle cells expressing tissue factor within the tunica media. The targeted emulsion increased the arterial wall gray scale (99.4 +/- 14.5; P < .05) relative to pretreatment (41.8 +/- 11.1, P < 0.05) and the control gray scale (pre-emulsion: 49.3 +/- 9.5; post-emulsion: 43.7 +/- 6.4; P < .05). The area of acoustic enhancement appeared to coincide with expression of induced tissue factor in the tunica media confirmed by immunohistochemistry. We have demonstrated that this novel nanoemulsion can infiltrate into arterial walls after balloon injury and localize the expression of overstretch-induced tissue factor within pig carotid arteries. Molecular imaging and quantification of complex, biochemical change, such as tissue factor expression after angioplasty, may prove to be a prognostically important predictor of subsequent restenosis. (J Am Soc Echocardiogr 2000;13:608-14.)}, author = {Lanza, Gregory M. and Abendschein, Dana R. and Hall, Christopher S. and Scott, Michael J. and Scherrer, David E. and Houseman, Andrew and Miller, James G. and Wickline, Samuel A. }, citeulike-article-id = {2460779}, doi = {10.1067/mje.2000.105840}, journal = {Journal of the American Society of Echocardiography}, keywords = {emulsions, imaging, nanotech, sccne}, month = {June}, number = {6}, pages = {608--614}, posted-at = {2008-03-03 12:36:36}, priority = {0}, title = {In Vivo Molecular Imaging of Stretch-Induced Tissue Factor in Carotid Arteries with Ligand-Targeted Nanoparticles}, url = {http://dx.doi.org/10.1067/mje.2000.105840}, volume = {13}, year = {2000} }

TY - JOUR ID - citeulike:2460779 L3 - citeulike-article-id:2460779 TI - In Vivo Molecular Imaging of Stretch-Induced Tissue Factor in Carotid Arteries with Ligand-Targeted Nanoparticles JF - Journal of the American Society of Echocardiography VL - 13 IS - 6 SP - 608 EP - 614 N2 - Molecular imaging permits tissues to be functionally characterized by identification of specific cell-surface receptors with targeted contrast agents. In our study, a ligand-targeted acoustic nanoparticle system was used to identify the angioplasty-induced expression of tissue factor by smooth muscle cells within the tunica media. Pig carotid arteries were overstretched bilaterally with balloon catheters, treated with a tissue factor-targeted or a control nanoparticle system, and imaged with intravascular ultrasound (20 MHz) before and after treatment. Carotid wall acoustic reflectivities were unaffected by overstretch injury. Tissue factor-targeted nanoemulsion bound and increased the echogenicity of smooth muscle cells expressing tissue factor within the tunica media. The targeted emulsion increased the arterial wall gray scale (99.4 +/- 14.5; P < .05) relative to pretreatment (41.8 +/- 11.1, P < 0.05) and the control gray scale (pre-emulsion: 49.3 +/- 9.5; post-emulsion: 43.7 +/- 6.4; P < .05). The area of acoustic enhancement appeared to coincide with expression of induced tissue factor in the tunica media confirmed by immunohistochemistry. We have demonstrated that this novel nanoemulsion can infiltrate into arterial walls after balloon injury and localize the expression of overstretch-induced tissue factor within pig carotid arteries. Molecular imaging and quantification of complex, biochemical change, such as tissue factor expression after angioplasty, may prove to be a prognostically important predictor of subsequent restenosis. (J Am Soc Echocardiogr 2000;13:608-14.) KW - emulsions KW - imaging KW - nanotech KW - sccne AU - Lanza, Gregory AU - Abendschein, Dana AU - Hall, Christopher AU - Scott, Michael AU - Scherrer, David AU - Houseman, Andrew AU - Miller, James AU - Wickline, Samuel PY - 2000/06// UR - http://dx.doi.org/10.1067/mje.2000.105840 ER -