Membrane Thinning Effect of Curcumin

@article{citeulike:2475405, abstract = {Interaction of curcumin with lipid bilayers is not well understood. A recent experiment showed that curcumin significantly affected the single channel lifetime of gramicidin in a DOPC bilayer without affecting its single channel conductance. We performed two experiments in order to understand this result. By isothermal titration calorimetry, we measured the partition coefficient of curcumin binding to DOPC bilayers. By X-ray lamellar diffraction, we measured the thickness change of DOPC bilayers as a function of curcumin-lipid ratio. A non-linear membrane thinning effect by curcumin was discovered. The gramicidin data were qualitatively interpreted by the combination of ITC and X-ray results. We show that curcumin not only thins the lipid bilayer, it might also weaken its elasticity moduli. The result implies that curcumin may affect the function of membrane proteins by modifying the properties of the host membrane. 10.1529/biophysj.107.126888}, author = {Hung, Wei-Chin and Chen, Fang-Yu and Lee, Chang-Chun and Sun, Yen and Lee, Ming-Tao and Huang, Huey W. }, citeulike-article-id = {2475405}, doi = {http://dx.doi.org/10.1529/biophysj.107.126888}, journal = {Biophys. J.}, keywords = {biomembranes, ion\_channels, ligand-membrane\_interactions}, month = {February}, pages = {biophysj.107.126888+}, posted-at = {2008-07-08 12:03:16}, priority = {0}, title = {Membrane Thinning Effect of Curcumin}, url = {http://dx.doi.org/10.1529/biophysj.107.126888}, year = {2008} }

TY - JOUR ID - citeulike:2475405 L3 - citeulike-article-id:2475405 TI - Membrane Thinning Effect of Curcumin JF - Biophys. J. SP - biophysj.107.126888 N2 - Interaction of curcumin with lipid bilayers is not well understood. A recent experiment showed that curcumin significantly affected the single channel lifetime of gramicidin in a DOPC bilayer without affecting its single channel conductance. We performed two experiments in order to understand this result. By isothermal titration calorimetry, we measured the partition coefficient of curcumin binding to DOPC bilayers. By X-ray lamellar diffraction, we measured the thickness change of DOPC bilayers as a function of curcumin-lipid ratio. A non-linear membrane thinning effect by curcumin was discovered. The gramicidin data were qualitatively interpreted by the combination of ITC and X-ray results. We show that curcumin not only thins the lipid bilayer, it might also weaken its elasticity moduli. The result implies that curcumin may affect the function of membrane proteins by modifying the properties of the host membrane. 10.1529/biophysj.107.126888 KW - biomembranes KW - ion_channels KW - ligand-membrane_interactions AU - Hung, Wei-Chin AU - Chen, Fang-Yu AU - Lee, Chang-Chun AU - Sun, Yen AU - Lee, Ming-Tao AU - Huang, Huey PY - 2008/02/29/ UR - http://dx.doi.org/10.1529/biophysj.107.126888 ER -