Charge-induced tilt in ordered-phase phosphatidylglycerol bilayers Evidence from x-ray diffraction Export

@article{citeulike:2743340, abstract = {X-ray diffraction studies have been performed, as a function of water content, on dipalmitoyl phosphatidyl-glycerol bilayers, both in the charged state at pH 8.0 and in the protonated state at pH 1.5, using buffers of 1.5 M salt concentration. Measurements were made at 20°C, and the high-angle reflections indicated that the bilayers were in the ordered phase at both pH values. Lamellar diffractions were observed under all conditions studied. The lamellar repeat reached a limiting value of 62.4 \r{A} (6.24 nm) at a water/lipid ratio of 0.24 at pH 8.0, and a limiting value of 67.3 \r{A} (6.73 nm) at a water/lipid ratio of 0.22 at pH 1.5. The area per lipid molecule in the plane of the bilayer, deduced from the bilayer thickness and the lipid partial specific volume, is 48 \r{A}2 (0.48 nm2) at pH 8.0 and 37 \r{A}2 (0.37 nm2) at pH 1.5. The area per molecule in the plane perpendicular to the chain axes, deduced from the X-ray short spacings, is 40.5 \r{A}2 (0.405 nm2) at pH 8.0 and 39.2 \r{A}2 (0.392 nm2) at pH 1.5. Thus the lipid molecules are tilted by approx. 30° relative to the bilayer normal at pH 8.0, but are not essentially untilted at pH 1.5.}, author = {Watts, Anthony and Harlos, Karl and Marsh, Derek}, citeulike-article-id = {2743340}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/0005-2736(81)90515-0}, citeulike-linkout-1 = {http://www.sciencedirect.com/science/article/B6T1T-47GHBXH-B3/1/fd5b447383b0c8b1b7659fb447be4b53}, day = {6}, doi = {10.1016/0005-2736(81)90515-0}, journal = {Biochimica et Biophysica Acta (BBA) - Biomembranes}, keywords = {cardiolipin, tilt}, month = {June}, number = {1}, pages = {91--96}, posted-at = {2008-05-01 20:49:39}, priority = {2}, title = {Charge-induced tilt in ordered-phase phosphatidylglycerol bilayers Evidence from x-ray diffraction}, url = {http://dx.doi.org/10.1016/0005-2736(81)90515-0}, volume = {645}, year = {1981} }

TY - JOUR ID - citeulike:2743340 L3 - citeulike-article-id:2743340 N2 - X-ray diffraction studies have been performed, as a function of water content, on dipalmitoyl phosphatidyl-glycerol bilayers, both in the charged state at pH 8.0 and in the protonated state at pH 1.5, using buffers of 1.5 M salt concentration. Measurements were made at 20°C, and the high-angle reflections indicated that the bilayers were in the ordered phase at both pH values. Lamellar diffractions were observed under all conditions studied. The lamellar repeat reached a limiting value of 62.4 Å (6.24 nm) at a water/lipid ratio of 0.24 at pH 8.0, and a limiting value of 67.3 Å (6.73 nm) at a water/lipid ratio of 0.22 at pH 1.5. The area per lipid molecule in the plane of the bilayer, deduced from the bilayer thickness and the lipid partial specific volume, is 48 Å2 (0.48 nm2) at pH 8.0 and 37 Å2 (0.37 nm2) at pH 1.5. The area per molecule in the plane perpendicular to the chain axes, deduced from the X-ray short spacings, is 40.5 Å2 (0.405 nm2) at pH 8.0 and 39.2 Å2 (0.392 nm2) at pH 1.5. Thus the lipid molecules are tilted by approx. 30° relative to the bilayer normal at pH 8.0, but are not essentially untilted at pH 1.5. IS - 1 JF - Biochimica et Biophysica Acta (BBA) - Biomembranes EP - 96 TI - Charge-induced tilt in ordered-phase phosphatidylglycerol bilayers Evidence from x-ray diffraction VL - 645 SP - 91 KW - cardiolipin KW - tilt AU - Watts, Anthony AU - Harlos, Karl AU - Marsh, Derek PY - 1981/06/06/ UR - http://dx.doi.org/10.1016/0005-2736(81)90515-0 ER -