Bending Stiffness of a Crystalline Actin Bundle Export

@article{Shin:04cc, abstract = {The acrosomal process of the sperm of the horseshoe crab ( Limulus polyphemus ) is a unique crystalline actin bundle, consisting of multiple actin filaments cross-linked by the actin-bundling protein, scruin. For successful fertilization, the acrosomal bundle must penetrate through a 30 μm thick jelly coat surrounding the egg and thus it must be sufficiently stiff. Here, we present two measurements of the bending stiffness of a single crystalline bundle of actin. Results from these measurements indicate that the actin:scruin composite bundle has an average elastic modulus of 2 GPa, which is similar to that of a single actin filament, and a bending stiffness that is more than two orders of magnitude larger than that of a bundle of uncross-linked actin filaments due to stiffening by the scruin matrix.}, author = {Shin, Jennifer H. and Mahadevan, L. and So, P. T. and Matsudaira, Paul}, citeulike-article-id = {4571297}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.jmb.2004.01.028}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0022283604000890}, day = {19}, doi = {10.1016/j.jmb.2004.01.028}, issn = {00222836}, journal = {Journal of Molecular Biology}, keywords = {actin, bundles}, month = {March}, number = {2}, pages = {255--261}, posted-at = {2009-05-21 18:08:59}, priority = {2}, title = {Bending Stiffness of a Crystalline Actin Bundle}, url = {http://dx.doi.org/10.1016/j.jmb.2004.01.028}, volume = {337}, year = {2004} }

TY - JOUR ID - Shin:04cc L3 - citeulike-article-id:4571297 N2 - The acrosomal process of the sperm of the horseshoe crab ( Limulus polyphemus ) is a unique crystalline actin bundle, consisting of multiple actin filaments cross-linked by the actin-bundling protein, scruin. For successful fertilization, the acrosomal bundle must penetrate through a 30 μm thick jelly coat surrounding the egg and thus it must be sufficiently stiff. Here, we present two measurements of the bending stiffness of a single crystalline bundle of actin. Results from these measurements indicate that the actin:scruin composite bundle has an average elastic modulus of 2 GPa, which is similar to that of a single actin filament, and a bending stiffness that is more than two orders of magnitude larger than that of a bundle of uncross-linked actin filaments due to stiffening by the scruin matrix. IS - 2 JF - Journal of Molecular Biology SN - 00222836 EP - 261 TI - Bending Stiffness of a Crystalline Actin Bundle VL - 337 SP - 255 KW - actin KW - bundles AU - Shin, Jennifer AU - Mahadevan, L AU - So, PT AU - Matsudaira, Paul PY - 2004/03/19/ UR - http://dx.doi.org/10.1016/j.jmb.2004.01.028 ER -