Association between Lifestyle Factors and CpG Island Methylation in a Cancer-Free Population. Export

@article{citeulike:6083191, abstract = {BACKGROUND: Many risk factors have been associated with cancer, such as age, family history, race, smoking, high-fat diet, and poor nutrition. It is important to reveal the molecular changes related to risk factors that could facilitate early detection, prevention, and overall control of cancer. METHODS: We selected six cancer-specific methylated genes that have previously been reported in primary tumors and have also been detected in different bodily fluids of cancer patients. Here, we used quantitative fluorogenic real-time methylation-specific PCR in plasma DNA samples for the detection of methylation changes from an asymptomatic population who do not have any known cancer. RESULTS: The promoter methylation frequencies of the studied genes were as follows: APC (7\%), CCND2 (22\%), GSTP1 (2\%), MGMT (9\%), RARbeta2 (29\%), and P16 (3\%). Promoter methylation of at least one of the genes analyzed was observed in approximately 46\% (72 of 157) of the samples by binary dichotomization. Promoter hypermethylation of at least two genes was detected in 17\% (26 of 157) of the samples. RARbeta2 methylation was observed in 45\% of subjects who had a high-fat diet in contrast with those who had a low-fat diet (23\%; P = 0.007). DISCUSSION: Our findings may help to elucidate early methylation changes that may lead to cancer development. These methylation changes could be due to exposure to risk factors and may be useful for cancer prevention measures such as changes in lifestyle. Longitudinal follow-up of a high-risk population is needed to understand the association of methylation of candidate genes in cancer development. (Cancer Epidemiol Biomarkers Prev 2009;18(11):2984-91).}, author = {Brait, Mariana and Ford, Jean G. and Papaiahgari, Srinivas and Garza, Mary A. and Lee, Jin I. and Loyo, Myriam and Maldonado, Leonel and Begum, Shahnaz and McCaffrey, Lee and Howerton, Mollie and Sidransky, David and Emerson, Mark R. and Ahmed, Saifuddin and Williams, Carla D. and Hoque, Mohammad Obaidul O.}, citeulike-article-id = {6083191}, citeulike-linkout-0 = {http://dx.doi.org/10.1158/1055-9965.EPI-08-1245}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19861513}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19861513}, day = {27}, doi = {10.1158/1055-9965.EPI-08-1245}, issn = {1538-7755}, journal = {Cancer epidemiology, biomarkers \& prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology}, keywords = {diet, lifestyle, methylation}, month = {October}, posted-at = {2009-11-07 18:06:34}, priority = {2}, title = {Association between Lifestyle Factors and CpG Island Methylation in a Cancer-Free Population.}, url = {http://dx.doi.org/10.1158/1055-9965.EPI-08-1245}, year = {2009} }

TY - JOUR ID - citeulike:6083191 L3 - citeulike-article-id:6083191 N2 - BACKGROUND: Many risk factors have been associated with cancer, such as age, family history, race, smoking, high-fat diet, and poor nutrition. It is important to reveal the molecular changes related to risk factors that could facilitate early detection, prevention, and overall control of cancer. METHODS: We selected six cancer-specific methylated genes that have previously been reported in primary tumors and have also been detected in different bodily fluids of cancer patients. Here, we used quantitative fluorogenic real-time methylation-specific PCR in plasma DNA samples for the detection of methylation changes from an asymptomatic population who do not have any known cancer. RESULTS: The promoter methylation frequencies of the studied genes were as follows: APC (7%), CCND2 (22%), GSTP1 (2%), MGMT (9%), RARbeta2 (29%), and P16 (3%). Promoter methylation of at least one of the genes analyzed was observed in approximately 46% (72 of 157) of the samples by binary dichotomization. Promoter hypermethylation of at least two genes was detected in 17% (26 of 157) of the samples. RARbeta2 methylation was observed in 45% of subjects who had a high-fat diet in contrast with those who had a low-fat diet (23%; P = 0.007). DISCUSSION: Our findings may help to elucidate early methylation changes that may lead to cancer development. These methylation changes could be due to exposure to risk factors and may be useful for cancer prevention measures such as changes in lifestyle. Longitudinal follow-up of a high-risk population is needed to understand the association of methylation of candidate genes in cancer development. (Cancer Epidemiol Biomarkers Prev 2009;18(11):2984-91). JF - Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology SN - 1538-7755 TI - Association between Lifestyle Factors and CpG Island Methylation in a Cancer-Free Population. KW - diet KW - lifestyle KW - methylation AU - Brait, Mariana AU - Ford, Jean AU - Papaiahgari, Srinivas AU - Garza, Mary AU - Lee, Jin AU - Loyo, Myriam AU - Maldonado, Leonel AU - Begum, Shahnaz AU - McCaffrey, Lee AU - Howerton, Mollie AU - Sidransky, David AU - Emerson, Mark AU - Ahmed, Saifuddin AU - Williams, Carla AU - Hoque, Mohammad Obaidul PY - 2009/10/27/ UR - http://dx.doi.org/10.1158/1055-9965.EPI-08-1245 ER -