Enhanced Yield of Neuroepithelial Precursors and Midbrain-Like Dopaminergic Neurons from Human Embryonic Stem Cells Using the BMP Antagonist Noggin.

@article{citeulike:972841, abstract = {It is currently not known whether dopamine (DA) neurons derived from human embryonic stem cells (hESCs) can survive in vivo and alleviate symptoms in models of Parkinson's disease (PD). Here, we report the use of Noggin (a bone-morphogenic protein antagonist) to induce neuroectodermal cell development and increase the yield of DA neurons from hESCs. A combination of stromal-derived inducing activity (SDIA) and Noggin markedly enhanced the generation of neuroepithelial progenitors that could give rise to DA neurons. In addition, Noggin diminished the occurrence of a fibroblast-like Nestin-positive precursor population that differentiated into myocytes. After transplantation of differentiated hESCs to a rodent model PD, some grafts contained human midbrain-like DA neurons. This protocol demonstrates hESC derivation and survival of hDA neurons appropriate for cell therapy in PD.}, address = {Center for Neuroregeneration Research, Udall Parkinson's Disease Center of Excellence, McLean Hospital, Harvard Medical School, Belmont, Massachusetts.}, author = {Sonntag, Kai-Christian C. and Pruszak, Jan and Yoshizaki, Takahito and van Arensbergen, Joris and Sanchez-Pernaute, Rosario and Isacson, Ole }, citeulike-article-id = {972841}, doi = {10.1634/stemcells.2006-0380}, issn = {1066-5099}, journal = {Stem Cells}, keywords = {noggin}, month = {October}, posted-at = {2006-12-04 05:53:43}, priority = {2}, title = {Enhanced Yield of Neuroepithelial Precursors and Midbrain-Like Dopaminergic Neurons from Human Embryonic Stem Cells Using the BMP Antagonist Noggin.}, url = {http://dx.doi.org/10.1634/stemcells.2006-0380}, year = {2006} }

TY - JOUR ID - citeulike:972841 L3 - citeulike-article-id:972841 TI - Enhanced Yield of Neuroepithelial Precursors and Midbrain-Like Dopaminergic Neurons from Human Embryonic Stem Cells Using the BMP Antagonist Noggin. JF - Stem Cells AD - Center for Neuroregeneration Research, Udall Parkinson's Disease Center of Excellence, McLean Hospital, Harvard Medical School, Belmont, Massachusetts. SN - 1066-5099 N2 - It is currently not known whether dopamine (DA) neurons derived from human embryonic stem cells (hESCs) can survive in vivo and alleviate symptoms in models of Parkinson's disease (PD). Here, we report the use of Noggin (a bone-morphogenic protein antagonist) to induce neuroectodermal cell development and increase the yield of DA neurons from hESCs. A combination of stromal-derived inducing activity (SDIA) and Noggin markedly enhanced the generation of neuroepithelial progenitors that could give rise to DA neurons. In addition, Noggin diminished the occurrence of a fibroblast-like Nestin-positive precursor population that differentiated into myocytes. After transplantation of differentiated hESCs to a rodent model PD, some grafts contained human midbrain-like DA neurons. This protocol demonstrates hESC derivation and survival of hDA neurons appropriate for cell therapy in PD. KW - noggin AU - Sonntag, Kai-Christian AU - Pruszak, Jan AU - Yoshizaki, Takahito AU - van Arensbergen, Joris AU - Sanchez-Pernaute, Rosario AU - Isacson, Ole PY - 2006/10/12/ UR - http://dx.doi.org/10.1634/stemcells.2006-0380 ER -