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SAXS–WAXS studies of the low-resolution structure in solution of xylose/glucose isomerase from Streptomyces rubiginosus

by: Maciej Kozak, Michał Taube
Radiation Physics and Chemistry, Vol. 78, No. 10. (30 October 2009), pp. S125-S128, doi:10.1016/j.radphyschem.2009.03.085  Key: citeulike:4982000

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Abstract

The structure and conformation of molecule of xylose/glucose isomerase from Streptomyces rubiginosus in solution (at pH 6 and 7.6; with and without the substrate) has been studied by small- and wide-angle scattering of synchrotron radiation (SAXS–WAXS). On the basis of the SAXS–WAXS data, the low-resolution structure in solution has been reconstructed using ab inito methods. A comparison of the models of glucose isomerase shows only small differences between the model in solution and the crystal structure.


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