Aspergillus niger genome-wide analysis reveals a large number of novel alpha-glucan acting enzymes with unexpected expression profiles. Export

@article{citeulike:2504288, abstract = {The filamentous ascomycete Aspergillus niger is well known for its ability to produce a large variety of enzymes for the degradation of plant polysaccharide material. A major carbon and energy source for this soil fungus is starch, which can be degraded by the concerted action of alpha-amylase, glucoamylase and alpha-glucosidase enzymes, members of the glycoside hydrolase (GH) families 13, 15 and 31, respectively. In this study we have combined analysis of the genome sequence of A. niger CBS 513.88 with microarray experiments to identify novel enzymes from these families and to predict their physiological functions. We have identified 17 previously unknown family GH13, 15 and 31 enzymes in the A. niger genome, all of which have orthologues in other aspergilli. Only two of the newly identified enzymes, a putative alpha-glucosidase (AgdB) and an alpha-amylase (AmyC), were predicted to play a role in starch degradation. The expression of the majority of the genes identified was not induced by maltose as carbon source, and not dependent on the presence of AmyR, the transcriptional regulator for starch degrading enzymes. The possible physiological functions of the other predicted family GH13, GH15 and GH31 enzymes, including intracellular enzymes and cell wall associated proteins, in alternative alpha-glucan modifying processes are discussed.}, address = {Clusius Laboratory, Molecular Microbiology and Kluyver Centre for Genomics of Industrial Fermentations, Institute of Biology Leiden, Leiden University, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands, xyuan@science.uva.nl.}, author = {Yuan, Xiao-Lian L. and van der Kaaij, Rachel M. and van den Hondel, Cees A. and Punt, Peter J. and van der Maarel, Marc J. and Dijkhuizen, Lubbert and Ram, Arthur F.}, citeulike-article-id = {2504288}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s00438-008-0332-7}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18320228}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18320228}, day = {5}, doi = {10.1007/s00438-008-0332-7}, issn = {1617-4615}, journal = {Mol Genet Genomics}, keywords = {alpha-glucosidase, alpha\_amylase, aspergillus, cell\_wall, cellulase, glucan}, month = {March}, posted-at = {2008-03-10 19:50:51}, priority = {2}, title = {Aspergillus niger genome-wide analysis reveals a large number of novel alpha-glucan acting enzymes with unexpected expression profiles.}, url = {http://dx.doi.org/10.1007/s00438-008-0332-7}, year = {2008} }

TY - JOUR ID - citeulike:2504288 L3 - citeulike-article-id:2504288 N2 - The filamentous ascomycete Aspergillus niger is well known for its ability to produce a large variety of enzymes for the degradation of plant polysaccharide material. A major carbon and energy source for this soil fungus is starch, which can be degraded by the concerted action of alpha-amylase, glucoamylase and alpha-glucosidase enzymes, members of the glycoside hydrolase (GH) families 13, 15 and 31, respectively. In this study we have combined analysis of the genome sequence of A. niger CBS 513.88 with microarray experiments to identify novel enzymes from these families and to predict their physiological functions. We have identified 17 previously unknown family GH13, 15 and 31 enzymes in the A. niger genome, all of which have orthologues in other aspergilli. Only two of the newly identified enzymes, a putative alpha-glucosidase (AgdB) and an alpha-amylase (AmyC), were predicted to play a role in starch degradation. The expression of the majority of the genes identified was not induced by maltose as carbon source, and not dependent on the presence of AmyR, the transcriptional regulator for starch degrading enzymes. The possible physiological functions of the other predicted family GH13, GH15 and GH31 enzymes, including intracellular enzymes and cell wall associated proteins, in alternative alpha-glucan modifying processes are discussed. JF - Mol Genet Genomics SN - 1617-4615 AD - Clusius Laboratory, Molecular Microbiology and Kluyver Centre for Genomics of Industrial Fermentations, Institute of Biology Leiden, Leiden University, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands, xyuan@science.uva.nl. TI - Aspergillus niger genome-wide analysis reveals a large number of novel alpha-glucan acting enzymes with unexpected expression profiles. KW - alpha-glucosidase KW - alpha_amylase KW - aspergillus KW - cell_wall KW - cellulase KW - glucan AU - Yuan, Xiao-Lian AU - van der Kaaij, Rachel AU - van den Hondel, Cees AU - Punt, Peter AU - van der Maarel, Marc AU - Dijkhuizen, Lubbert AU - Ram, Arthur PY - 2008/03/05/ UR - http://dx.doi.org/10.1007/s00438-008-0332-7 ER -