The identification of a novel Pleurotus ostreatus dsRNA virus and determination of the distribution of viruses in mushroom spores. Export

@article{citeulike:2811974, abstract = {Double-stranded RNAs and virus particles were identified in Pleurotus ostreatus strain Shin-Nong in Korea. Isometric virus particles with a diameter of 33 nm were purified, which are similar to other Pleurotus viruses reported previously. This strain contains 5 dsRNAs, 8.0, 2.5, 2.4, 2.0, and 1.8 kb in size. The virus particles contain 2 dsRNAs, designated RNA-1 (2.5 kb), and RNA-2 (2.4 kb) which is a typical pattern of Partitiviridae. A non-encapsidated dsRNA of about 8.0 kb also was identified. Partial cDNA from RNA-1 was cloned, and sequence analysis revealed that this gene codes for RdRp. The comparison of the sequence from partial cDNA clone showed 35\% amino acid homology with the C-terminal end of the RdRp gene of Helicobasidum mompa virus and Rosalinia necatrix virus. Specific primers designed from the partial sequences successfully amplified RT-PCR product from the infected mycelium and a single spore culture. We used these primers to determine the pattern of distribution of viruses in spores. Of the 96 different single spore cultures generated from Shin-Nong strain, a specific RT-PCR product was identified in 25 cultures, indicating that about 26\% of basidiospores contain viruses.}, address = {Department of Molecular Bioscience, Division of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea.}, author = {Kim, Y. J. and Kim, J. Y. and Kim, J. H. and Yoon, S. M. and Yoo, Y. B. and Yie, S. W.}, citeulike-article-id = {2811974}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/18337700}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=18337700}, issn = {1225-8873}, journal = {Journal of microbiology (Seoul, Korea)}, keywords = {basidiomycota, dsrna, fungi, mycovirus, rna\_silencing, rnaworld, virus}, month = {February}, number = {1}, pages = {95--99}, posted-at = {2008-05-19 06:46:30}, priority = {2}, title = {The identification of a novel Pleurotus ostreatus dsRNA virus and determination of the distribution of viruses in mushroom spores.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/18337700}, volume = {46}, year = {2008} }

TY - JOUR ID - citeulike:2811974 L3 - citeulike-article-id:2811974 N2 - Double-stranded RNAs and virus particles were identified in Pleurotus ostreatus strain Shin-Nong in Korea. Isometric virus particles with a diameter of 33 nm were purified, which are similar to other Pleurotus viruses reported previously. This strain contains 5 dsRNAs, 8.0, 2.5, 2.4, 2.0, and 1.8 kb in size. The virus particles contain 2 dsRNAs, designated RNA-1 (2.5 kb), and RNA-2 (2.4 kb) which is a typical pattern of Partitiviridae. A non-encapsidated dsRNA of about 8.0 kb also was identified. Partial cDNA from RNA-1 was cloned, and sequence analysis revealed that this gene codes for RdRp. The comparison of the sequence from partial cDNA clone showed 35% amino acid homology with the C-terminal end of the RdRp gene of Helicobasidum mompa virus and Rosalinia necatrix virus. Specific primers designed from the partial sequences successfully amplified RT-PCR product from the infected mycelium and a single spore culture. We used these primers to determine the pattern of distribution of viruses in spores. Of the 96 different single spore cultures generated from Shin-Nong strain, a specific RT-PCR product was identified in 25 cultures, indicating that about 26% of basidiospores contain viruses. IS - 1 JF - Journal of microbiology (Seoul, Korea) SN - 1225-8873 AD - Department of Molecular Bioscience, Division of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea. EP - 99 TI - The identification of a novel Pleurotus ostreatus dsRNA virus and determination of the distribution of viruses in mushroom spores. VL - 46 SP - 95 KW - basidiomycota KW - dsrna KW - fungi KW - mycovirus KW - rna_silencing KW - rnaworld KW - virus AU - Kim, YJ AU - Kim, JY AU - Kim, JH AU - Yoon, SM AU - Yoo, YB AU - Yie, SW PY - 2008/02// UR - http://view.ncbi.nlm.nih.gov/pubmed/18337700 ER -