Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity Export

@article{citeulike:2742584, abstract = {The dynamic ability of neuronal dendrites to shape and integrate synaptic responses is the hallmark of information processing in the brain. Effectively studying this phenomenon requires concurrent measurements at multiple sites on live neurons. Substantial progress has been made by optical imaging systems that combine confocal and multiphoton microscopy with inertia-free laser scanning. However, all of the systems developed so far restrict fast imaging to two dimensions. This severely limits the extent to which neurons can be studied, as they represent complex three-dimensional structures. Here we present a new imaging system that utilizes a unique arrangement of acousto-optic deflectors to steer a focused, ultra-fast laser beam to arbitrary locations in three-dimensional space without moving the objective lens. As we demonstrate, this highly versatile random-access multiphoton microscope supports functional imaging of complex three-dimensional cellular structures such as neuronal dendrites or neural populations at acquisition rates on the order of tens of kilohertz.}, address = {[1] Department of Bioengineering, Rice University, 6100 Main Street, Suite 116 Keck Hall, Houston, Texas 77005, USA. [2] Department of Neuroscience, Baylor College of Medicine, One Baylor Plaza S730, Houston, Texas 77030, USA.}, author = {Duemani Reddy, Gaddum and Kelleher, Keith and Fink, Rudy and Saggau, Peter}, booktitle = {Nat Neurosci}, citeulike-article-id = {2742584}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nn.2116}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nn.2116}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18432198}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18432198}, day = {27}, doi = {10.1038/nn.2116}, issn = {1097-6256}, journal = {Nature Neuroscience}, keywords = {2pe, aod, ca\_phys, method}, month = {April}, number = {6}, pages = {713--720}, posted-at = {2008-06-06 13:49:15}, priority = {2}, publisher = {Nature Publishing Group}, title = {Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity}, url = {http://dx.doi.org/10.1038/nn.2116}, volume = {11}, year = {2008} }

TY - JOUR ID - citeulike:2742584 L3 - citeulike-article-id:2742584 N2 - The dynamic ability of neuronal dendrites to shape and integrate synaptic responses is the hallmark of information processing in the brain. Effectively studying this phenomenon requires concurrent measurements at multiple sites on live neurons. Substantial progress has been made by optical imaging systems that combine confocal and multiphoton microscopy with inertia-free laser scanning. However, all of the systems developed so far restrict fast imaging to two dimensions. This severely limits the extent to which neurons can be studied, as they represent complex three-dimensional structures. Here we present a new imaging system that utilizes a unique arrangement of acousto-optic deflectors to steer a focused, ultra-fast laser beam to arbitrary locations in three-dimensional space without moving the objective lens. As we demonstrate, this highly versatile random-access multiphoton microscope supports functional imaging of complex three-dimensional cellular structures such as neuronal dendrites or neural populations at acquisition rates on the order of tens of kilohertz. IS - 6 JF - Nature Neuroscience SN - 1097-6256 AD - [1] Department of Bioengineering, Rice University, 6100 Main Street, Suite 116 Keck Hall, Houston, Texas 77005, USA. [2] Department of Neuroscience, Baylor College of Medicine, One Baylor Plaza S730, Houston, Texas 77030, USA. EP - 720 TI - Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity PB - Nature Publishing Group VL - 11 T2 - Nat Neurosci SP - 713 KW - 2pe KW - aod KW - ca_phys KW - method AU - Duemani Reddy, Gaddum AU - Kelleher, Keith AU - Fink, Rudy AU - Saggau, Peter PY - 2008/04/27/ UR - http://dx.doi.org/10.1038/nn.2116 ER -