Computational Analysis of Action Potential Initiation in Mitral Cell Soma and Dendrites Based on Dual Patch Recordings Export

@article{citeulike:4210127, abstract = {Shen, Gongyu Y., Wei R. Chen, Jens Midtgaard, Gordon M. Shepherd, and Michael L. Hines. Computational Analysis of Action Potential Initiation in Mitral Cell Soma and Dendrites Based on Dual Patch Recordings. J. Neurophysiol. 82: 3006-3020, 1999. In olfactory mitral cells, dual patch recordings show that the site of action potential initiation can shift between soma and distal primary dendrite and that the shift is dependent on the location and strength of electrode current injection. We have analyzed the mechanisms underlying this shift, using a model of the mitral cell that takes advantage of the constraints available from the two recording sites. Starting with homogeneous Hodgkin-Huxley-like Na+-K+ channel distribution in the soma-dendritic region and much higher sodium channel density in the axonal region, the model's channel kinetics and density were adjusted by a fitting algorithm so that the model response was virtually identical to the experimental data. The combination of loading effects and much higher sodium channel density in the axon relative to the soma-dendritic region results in significantly lower "voltage threshold" for action potential initiation in the axon; the axon therefore fires first unless the voltage gradient in the primary dendrite is steep enough for it to reach its higher threshold. The results thus provide a quantitative explanation for the stimulus strength and position dependence of the site of action potential initiation in the mitral cell.}, author = {Shen, Gongyu Y. and Chen, Wei R. and Midtgaard, Jens and Shepherd, Gordon M. and Hines, Michael L.}, citeulike-article-id = {4210127}, citeulike-linkout-0 = {http://jn.physiology.org/cgi/content/abstract/82/6/3006}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/10601436}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=10601436}, day = {1}, journal = {J Neurophysiol}, keywords = {carnevale, computational, olfaction, qual, spike\_initiation}, month = {December}, number = {6}, pages = {3006--3020}, posted-at = {2009-03-23 14:52:06}, priority = {2}, title = {Computational Analysis of Action Potential Initiation in Mitral Cell Soma and Dendrites Based on Dual Patch Recordings}, url = {http://jn.physiology.org/cgi/content/abstract/82/6/3006}, volume = {82}, year = {1999} }

TY - JOUR ID - citeulike:4210127 L3 - citeulike-article-id:4210127 N2 - Shen, Gongyu Y., Wei R. Chen, Jens Midtgaard, Gordon M. Shepherd, and Michael L. Hines. Computational Analysis of Action Potential Initiation in Mitral Cell Soma and Dendrites Based on Dual Patch Recordings. J. Neurophysiol. 82: 3006-3020, 1999. In olfactory mitral cells, dual patch recordings show that the site of action potential initiation can shift between soma and distal primary dendrite and that the shift is dependent on the location and strength of electrode current injection. We have analyzed the mechanisms underlying this shift, using a model of the mitral cell that takes advantage of the constraints available from the two recording sites. Starting with homogeneous Hodgkin-Huxley-like Na+-K+ channel distribution in the soma-dendritic region and much higher sodium channel density in the axonal region, the model's channel kinetics and density were adjusted by a fitting algorithm so that the model response was virtually identical to the experimental data. The combination of loading effects and much higher sodium channel density in the axon relative to the soma-dendritic region results in significantly lower "voltage threshold" for action potential initiation in the axon; the axon therefore fires first unless the voltage gradient in the primary dendrite is steep enough for it to reach its higher threshold. The results thus provide a quantitative explanation for the stimulus strength and position dependence of the site of action potential initiation in the mitral cell. IS - 6 JF - J Neurophysiol EP - 3020 TI - Computational Analysis of Action Potential Initiation in Mitral Cell Soma and Dendrites Based on Dual Patch Recordings VL - 82 SP - 3006 KW - carnevale KW - computational KW - olfaction KW - qual KW - spike_initiation AU - Shen, Gongyu AU - Chen, Wei AU - Midtgaard, Jens AU - Shepherd, Gordon AU - Hines, Michael PY - 1999/12/01/ UR - http://jn.physiology.org/cgi/content/abstract/82/6/3006 ER -