Libraries against libraries for combinatorial selection of replicating antigen-antibody pairs. Export

@article{citeulike:4002604, abstract = {Antibodies are among the most highly selective tight-binding ligands for proteins. Because the human genome project has deciphered the proteome, there is an opportunity to use combinatorial antibody libraries to select high-affinity antibodies to every protein encoded by the genome. However, this is a large task because the selection formats used today for combinatorial antibody libraries are geared toward generating antibodies to one antigen at a time. Here, we describe a method that accelerates the identification of antibodies to a multitude of antigens simultaneously by matching combinatorial antibody libraries against eukaryotic antigen libraries so that replication-competent cognate antigen-antibody pairs can be directly selected. Phage and yeast display systems are used because they each link genotype to phenotype and can be replicated individually. When combined with cell sorting, the two libraries can be selected against each other for recovery of cognate antigen-antibody clones in a single experiment.}, author = {Bowley, Diana R R. and Jones, Teresa M M. and Burton, Dennis R R. and Lerner, Richard A A.}, citeulike-article-id = {4002604}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0812291106}, citeulike-linkout-1 = {http://www.pnas.org/content/106/5/1380.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/106/5/1380.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19139405}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19139405}, day = {12}, doi = {10.1073/pnas.0812291106}, issn = {1091-6490}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {biochemistry, combinatorial, genome}, month = {January}, posted-at = {2009-02-03 22:53:01}, priority = {2}, title = {Libraries against libraries for combinatorial selection of replicating antigen-antibody pairs.}, url = {http://dx.doi.org/10.1073/pnas.0812291106}, year = {2009} }

TY - JOUR ID - citeulike:4002604 L3 - citeulike-article-id:4002604 N2 - Antibodies are among the most highly selective tight-binding ligands for proteins. Because the human genome project has deciphered the proteome, there is an opportunity to use combinatorial antibody libraries to select high-affinity antibodies to every protein encoded by the genome. However, this is a large task because the selection formats used today for combinatorial antibody libraries are geared toward generating antibodies to one antigen at a time. Here, we describe a method that accelerates the identification of antibodies to a multitude of antigens simultaneously by matching combinatorial antibody libraries against eukaryotic antigen libraries so that replication-competent cognate antigen-antibody pairs can be directly selected. Phage and yeast display systems are used because they each link genotype to phenotype and can be replicated individually. When combined with cell sorting, the two libraries can be selected against each other for recovery of cognate antigen-antibody clones in a single experiment. JF - Proceedings of the National Academy of Sciences of the United States of America SN - 1091-6490 TI - Libraries against libraries for combinatorial selection of replicating antigen-antibody pairs. KW - biochemistry KW - combinatorial KW - genome AU - Bowley, Diana R AU - Jones, Teresa M AU - Burton, Dennis R AU - Lerner, Richard A PY - 2009/01/12/ UR - http://dx.doi.org/10.1073/pnas.0812291106 ER -