Engineering Entropy-Driven Reactions and Networks Catalyzed by DNA Export

@article{citeulike:1942937, abstract = {Artificial biochemical circuits are likely to play as large a role in biological engineering as electrical circuits have played in the engineering of electromechanical devices. Toward that end, nucleic acids provide a designable substrate for the regulation of biochemical reactions. However, it has been difficult to incorporate signal amplification components. We introduce a design strategy that allows a specified input oligonucleotide to catalyze the release of a specified output oligonucleotide, which in turn can serve as a catalyst for other reactions. This reaction, which is driven forward by the configurational entropy of the released molecule, provides an amplifying circuit element that is simple, fast, modular, composable, and robust. We have constructed and characterized several circuits that amplify nucleic acid signals, including a feedforward cascade with quadratic kinetics and a positive feedback circuit with exponential growth kinetics. 10.1126/science.1148532}, author = {Zhang, David Y. and Turberfield, Andrew J. and Yurke, Bernard and Winfree, Erik}, citeulike-article-id = {1942937}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1148532}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/318/5853/1121}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18006742}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18006742}, day = {16}, doi = {10.1126/science.1148532}, journal = {Science}, keywords = {circuits, dna, logic, networks}, month = {November}, number = {5853}, pages = {1121--1125}, posted-at = {2008-01-21 19:06:07}, priority = {2}, title = {Engineering Entropy-Driven Reactions and Networks Catalyzed by DNA}, url = {http://dx.doi.org/10.1126/science.1148532}, volume = {318}, year = {2007} }

TY - JOUR ID - citeulike:1942937 L3 - citeulike-article-id:1942937 N2 - Artificial biochemical circuits are likely to play as large a role in biological engineering as electrical circuits have played in the engineering of electromechanical devices. Toward that end, nucleic acids provide a designable substrate for the regulation of biochemical reactions. However, it has been difficult to incorporate signal amplification components. We introduce a design strategy that allows a specified input oligonucleotide to catalyze the release of a specified output oligonucleotide, which in turn can serve as a catalyst for other reactions. This reaction, which is driven forward by the configurational entropy of the released molecule, provides an amplifying circuit element that is simple, fast, modular, composable, and robust. We have constructed and characterized several circuits that amplify nucleic acid signals, including a feedforward cascade with quadratic kinetics and a positive feedback circuit with exponential growth kinetics. 10.1126/science.1148532 IS - 5853 JF - Science EP - 1125 TI - Engineering Entropy-Driven Reactions and Networks Catalyzed by DNA VL - 318 SP - 1121 KW - circuits KW - dna KW - logic KW - networks AU - Zhang, David AU - Turberfield, Andrew AU - Yurke, Bernard AU - Winfree, Erik PY - 2007/11/16/ UR - http://dx.doi.org/10.1126/science.1148532 ER -