Development of input connections in neural cultures. Export

@article{citeulike:3201278, abstract = {We introduce an approach for the quantitative assessment of the connectivity in neuronal cultures, based on the statistical mechanics of percolation on a graph. This allows us to monitor the development of the culture and to see the emergence of connectivity in the network. The culture becomes fully connected at a time equivalent to the expected time of birth. The spontaneous bursting activity that characterizes cultures develops in parallel with the connectivity. The average number of inputs per neuron can be quantitatively determined in units of m(0), the number of activated inputs needed to excite the neuron. For m(0) approximately 15 we find that hippocampal neurons have on average approximately 60-120 inputs, whereas cortical neurons have approximately 75-150, depending on neuronal density. The ratio of excitatory to inhibitory neurons is determined by using the GABA(A) antagonist bicuculine. This ratio changes during development and reaches the final value at day 7-8, coinciding with the expected time of the GABA switch. For hippocampal cultures the inhibitory cells comprise approximately 30\% of the neurons in the culture whereas for cortical cultures they are approximately 20\%. Such detailed global information on the connectivity of networks in neuronal cultures is at present inaccessible by any electrophysiological or other technique.}, address = {Department of Physics of Complex Systems, Weizmann Institute of Science, Rehovot 76100, Israel.}, author = {Soriano, Jordi and Rodr\'{\i}guez Mart\'{\i}nez, Mar\'{\i}a and Tlusty, Tsvi and Moses, Elisha}, citeulike-article-id = {3201278}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0707492105}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18772389}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18772389}, day = {4}, doi = {10.1073/pnas.0707492105}, issn = {1091-6490}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {connectivity, gaba}, month = {September}, posted-at = {2008-09-07 22:20:16}, priority = {2}, title = {Development of input connections in neural cultures.}, url = {http://dx.doi.org/10.1073/pnas.0707492105}, year = {2008} }

TY - JOUR ID - citeulike:3201278 L3 - citeulike-article-id:3201278 N2 - We introduce an approach for the quantitative assessment of the connectivity in neuronal cultures, based on the statistical mechanics of percolation on a graph. This allows us to monitor the development of the culture and to see the emergence of connectivity in the network. The culture becomes fully connected at a time equivalent to the expected time of birth. The spontaneous bursting activity that characterizes cultures develops in parallel with the connectivity. The average number of inputs per neuron can be quantitatively determined in units of m(0), the number of activated inputs needed to excite the neuron. For m(0) approximately 15 we find that hippocampal neurons have on average approximately 60-120 inputs, whereas cortical neurons have approximately 75-150, depending on neuronal density. The ratio of excitatory to inhibitory neurons is determined by using the GABA(A) antagonist bicuculine. This ratio changes during development and reaches the final value at day 7-8, coinciding with the expected time of the GABA switch. For hippocampal cultures the inhibitory cells comprise approximately 30% of the neurons in the culture whereas for cortical cultures they are approximately 20%. Such detailed global information on the connectivity of networks in neuronal cultures is at present inaccessible by any electrophysiological or other technique. JF - Proceedings of the National Academy of Sciences of the United States of America SN - 1091-6490 AD - Department of Physics of Complex Systems, Weizmann Institute of Science, Rehovot 76100, Israel. TI - Development of input connections in neural cultures. KW - connectivity KW - gaba AU - Soriano, Jordi AU - Rodríguez Martínez, María AU - Tlusty, Tsvi AU - Moses, Elisha PY - 2008/09/04/ UR - http://dx.doi.org/10.1073/pnas.0707492105 ER -