The membrane-proximal scavenger receptor cysteine-rich domain of CD6 contains the activated leukocyte cell adhesion molecule binding site. Export

@article{citeulike:562085, abstract = {Binding studies with a CD6 immunoglobulin fusion protein (CD6 Rg) resulted in the identification and cloning of a CD6 ligand. This ligand was found to be a member of the immunoglobulin supergene family and was named ALCAM (activated leukocyte cell adhesion molecule). Cell adhesion assays showed that CD6-ALCAM interactions mediate thymocyte-thymic epithelium cell binding. ALCAM is also expressed by activated leukocytes and neurons and may be involved in interactions between T cells and activated leukocytes and between cells of the immune and nervous systems, respectively. Herein we describe the preparation of domain-specific murine CD6 Rg fusion proteins and show that the membrane-proximal SRCR (scavenger receptor cysteine-rich) domain of CD6 contains the ALCAM binding site. We also show that mAbs which bind to this domain preferentially block CD6-ALCAM binding. These results demonstrate that the membrane-proximal SRCR domain of CD6 is necessary for CD6 binding to ALCAM and provide the first direct evidence for the interaction of an SRCR domain with a ligand.}, address = {Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, Washington 98121, USA.}, author = {Whitney, G. S. and Starling, G. C. and Bowen, M. A. and Modrell, B. and Siadak, A. W. and Aruffo, A.}, citeulike-article-id = {562085}, citeulike-linkout-0 = {http://dx.doi.org/10.1074/jbc.270.31.18187}, citeulike-linkout-1 = {http://www.jbc.org/cgi/content/abstract/270/31/18187}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/7543097}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=7543097}, day = {4}, doi = {10.1074/jbc.270.31.18187}, issn = {0021-9258}, journal = {J Biol Chem}, keywords = {alcam, cd6, ligand}, month = {August}, number = {31}, pages = {18187--18190}, posted-at = {2006-03-24 08:57:05}, priority = {0}, title = {The membrane-proximal scavenger receptor cysteine-rich domain of CD6 contains the activated leukocyte cell adhesion molecule binding site.}, url = {http://dx.doi.org/10.1074/jbc.270.31.18187}, volume = {270}, year = {1995} }

TY - JOUR ID - citeulike:562085 L3 - citeulike-article-id:562085 N2 - Binding studies with a CD6 immunoglobulin fusion protein (CD6 Rg) resulted in the identification and cloning of a CD6 ligand. This ligand was found to be a member of the immunoglobulin supergene family and was named ALCAM (activated leukocyte cell adhesion molecule). Cell adhesion assays showed that CD6-ALCAM interactions mediate thymocyte-thymic epithelium cell binding. ALCAM is also expressed by activated leukocytes and neurons and may be involved in interactions between T cells and activated leukocytes and between cells of the immune and nervous systems, respectively. Herein we describe the preparation of domain-specific murine CD6 Rg fusion proteins and show that the membrane-proximal SRCR (scavenger receptor cysteine-rich) domain of CD6 contains the ALCAM binding site. We also show that mAbs which bind to this domain preferentially block CD6-ALCAM binding. These results demonstrate that the membrane-proximal SRCR domain of CD6 is necessary for CD6 binding to ALCAM and provide the first direct evidence for the interaction of an SRCR domain with a ligand. IS - 31 JF - J Biol Chem SN - 0021-9258 AD - Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, Washington 98121, USA. EP - 18190 TI - The membrane-proximal scavenger receptor cysteine-rich domain of CD6 contains the activated leukocyte cell adhesion molecule binding site. VL - 270 SP - 18187 KW - alcam KW - cd6 KW - ligand AU - Whitney, GS AU - Starling, GC AU - Bowen, MA AU - Modrell, B AU - Siadak, AW AU - Aruffo, A PY - 1995/08/04/ UR - http://dx.doi.org/10.1074/jbc.270.31.18187 ER -