The accessory molecules CD5 and CD6 associate on the membrane of lymphoid T cells. Export

@article{citeulike:562086, abstract = {CD5 and CD6 are closely related lymphocyte surface receptors of the scavenger receptor cysteine-rich superfamily, which show highly homologous extracellular regions but little conserved cytoplasmic tails. Both molecules are expressed on the same lymphocyte populations (thymocytes, mature T cells, and B1a cells) and share similar co-stimulatory properties on mature T cells. Although several works have been reported on the molecular associations and the signaling pathway mediated by CD5, very limited information is available for CD6 in this regard. Here we show the physical association of CD5 and CD6 at the cell membrane of lymphocytes, as well as their localization at the immunological synapse. CD5 and CD6 co-immunoprecipitate from Brij 96 but not Nonidet P-40 cell lysates, independently of both the co-expression of other lymphocyte surface receptors and the integrity of CD5 cytoplasmic region. Fluorescence resonance energy transfer analysis, co-capping, and co-modulation experiments demonstrate the physical in vivo association of CD5 and CD6. Analysis of T cell/antigen-presenting cells conjugates shows the accumulation of both molecules at the immunological synapse. These results indicate that CD5 and CD6 are structurally and physically related receptors, which may be functionally linked to provide either similar or complementary accessory signals during T cell activation and/or differentiation.}, address = {Servei d'Immunologia, Institut Clinic d'Infeccions i Immunologia, Institut d'Investigacions Biom\`{e}diques August Pi i Sunyer, Hospital Clinic, Barcelona 08036, Spain.}, author = {Gimferrer, I. and Farn\'{o}s, M. and Calvo, M. and Mittelbrunn, M. and Enrich, C. and S\'{a}nchez-Madrid, F. and Vives, J. and Lozano, F.}, citeulike-article-id = {562086}, citeulike-linkout-0 = {http://dx.doi.org/10.1074/jbc.M209591200}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/12473675}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=12473675}, day = {7}, doi = {10.1074/jbc.M209591200}, issn = {0021-9258}, journal = {J Biol Chem}, keywords = {cd5, cd6, cell, t}, month = {March}, number = {10}, pages = {8564--8571}, posted-at = {2006-03-24 08:58:03}, priority = {0}, title = {The accessory molecules CD5 and CD6 associate on the membrane of lymphoid T cells.}, url = {http://dx.doi.org/10.1074/jbc.M209591200}, volume = {278}, year = {2003} }

TY - JOUR ID - citeulike:562086 L3 - citeulike-article-id:562086 N2 - CD5 and CD6 are closely related lymphocyte surface receptors of the scavenger receptor cysteine-rich superfamily, which show highly homologous extracellular regions but little conserved cytoplasmic tails. Both molecules are expressed on the same lymphocyte populations (thymocytes, mature T cells, and B1a cells) and share similar co-stimulatory properties on mature T cells. Although several works have been reported on the molecular associations and the signaling pathway mediated by CD5, very limited information is available for CD6 in this regard. Here we show the physical association of CD5 and CD6 at the cell membrane of lymphocytes, as well as their localization at the immunological synapse. CD5 and CD6 co-immunoprecipitate from Brij 96 but not Nonidet P-40 cell lysates, independently of both the co-expression of other lymphocyte surface receptors and the integrity of CD5 cytoplasmic region. Fluorescence resonance energy transfer analysis, co-capping, and co-modulation experiments demonstrate the physical in vivo association of CD5 and CD6. Analysis of T cell/antigen-presenting cells conjugates shows the accumulation of both molecules at the immunological synapse. These results indicate that CD5 and CD6 are structurally and physically related receptors, which may be functionally linked to provide either similar or complementary accessory signals during T cell activation and/or differentiation. IS - 10 JF - J Biol Chem SN - 0021-9258 AD - Servei d'Immunologia, Institut Clinic d'Infeccions i Immunologia, Institut d'Investigacions Biomèdiques August Pi i Sunyer, Hospital Clinic, Barcelona 08036, Spain. EP - 8571 TI - The accessory molecules CD5 and CD6 associate on the membrane of lymphoid T cells. VL - 278 SP - 8564 KW - cd5 KW - cd6 KW - cell KW - t AU - Gimferrer, I AU - Farnós, M AU - Calvo, M AU - Mittelbrunn, M AU - Enrich, C AU - Sánchez-Madrid, F AU - Vives, J AU - Lozano, F PY - 2003/03/07/ UR - http://dx.doi.org/10.1074/jbc.M209591200 ER -