Inoculation of a nonhost tissue culture with Cronartium ribicola
Cultured tissues from Pseudotsuga menziesii (Mirb.) Franco were successfully inoculated with Cronartium ribicola J. C. Fisch. ex Rabenh. Typical intracellular haustoria were formed 60 days after inoculation. Invaded nonhost tissues were necrotic within 90 days, and dead within 120 days. Tissue cultures of either Pinus monticola Dougl. or P. menziesii had an attractant effect on nearby rust mycelium.