Procedures and media for obtaining tissue cultures of 12 conifer species
Procedures for obtaining axenic tissue cultures of Abies grandis, Larix occidentalis, Picea abies, Picea engelmanni, Picea pungens, Pinus albicaulis, Pinus contorta, Pinus flexilis, Pinus nigra, Pinus ponderosa, Pseudotsuga menziesii, and Thuja plicata are described. All species were cultured on a basal medium containing calcium nitrate, magnesium sulphate, potassium phosphate (monobasic), ammonium sulfate, ferric sulfate, manganese sulfate, glucose, 0.8% Bacto agar, and one of three auxins (indoleacetic acid (IAA), naphthaleneacetic acid (NAA), or 2,4-dichlorophenoxyacetic acid (2,4-D)) at various concentrations. Growth of most species was further enhanced by the addition of several vitamins and amino acids. These species were relatively specific in their auxin requirements. The type of sterilant (H2O2, NaOCl) and manipulation required in the preparatory procedures were also specific for individual species.