Molecular Mechanism of the Nacreous Layer Formation in Pinctada maxima Export

@article{citeulike:2925792, abstract = {We have cloned the cDNAs that encode two kinds of molluscan shell matrix proteins, namely N66 and N14, in the nacreous layer of Pinctada maxima. N66 is composed of carbonic anhydrase-like and repeat domains, as described for nacrein (1) in the pearls of P. fucata. N14 is homologous to N16, recently found in the nacreous layer of P. fucata (2) and is characterized by high proportions of Gly, Tyr, and Asn together with NG repeat sequences. The molecular weights of these proteins were estimated as 59,814 and 13,734 Da, respectively. Structural differences were clearly indicated in the alignment and length of the repeat sequences of the sets of the homogeneous proteins (N66/nacrein and N14/N16). The longer repeat sequences of N66 and N14 may be responsible for P. maxima's excellent property of calcification. The in vitro crystallization experiments revealed that the mixture of N66 and N14 could induce platy aragonite layers highly similar to the nacreous layer, once adsorbed onto the membrane of the water-insoluble matrix.}, author = {Kono, M. and Hayashi, N. and Samata, T.}, citeulike-article-id = {2925792}, citeulike-linkout-0 = {http://dx.doi.org/10.1006/bbrc.2000.2274}, citeulike-linkout-1 = {http://www.ingentaconnect.com/content/els/0006291x/2000/00000269/00000001/art92274}, doi = {10.1006/bbrc.2000.2274}, issn = {0006-291X}, journal = {Biochemical and Biophysical Research Communications}, keywords = {cdna, pincatda}, month = {March}, pages = {213--218}, posted-at = {2008-06-25 13:03:20}, priority = {2}, publisher = {Elsevier}, title = {Molecular Mechanism of the Nacreous Layer Formation in Pinctada maxima}, url = {http://dx.doi.org/10.1006/bbrc.2000.2274}, year = {2000} }

TY - JOUR ID - citeulike:2925792 L3 - citeulike-article-id:2925792 N2 - We have cloned the cDNAs that encode two kinds of molluscan shell matrix proteins, namely N66 and N14, in the nacreous layer of Pinctada maxima. N66 is composed of carbonic anhydrase-like and repeat domains, as described for nacrein (1) in the pearls of P. fucata. N14 is homologous to N16, recently found in the nacreous layer of P. fucata (2) and is characterized by high proportions of Gly, Tyr, and Asn together with NG repeat sequences. The molecular weights of these proteins were estimated as 59,814 and 13,734 Da, respectively. Structural differences were clearly indicated in the alignment and length of the repeat sequences of the sets of the homogeneous proteins (N66/nacrein and N14/N16). The longer repeat sequences of N66 and N14 may be responsible for P. maxima's excellent property of calcification. The in vitro crystallization experiments revealed that the mixture of N66 and N14 could induce platy aragonite layers highly similar to the nacreous layer, once adsorbed onto the membrane of the water-insoluble matrix. JF - Biochemical and Biophysical Research Communications SN - 0006-291X EP - 218 TI - Molecular Mechanism of the Nacreous Layer Formation in Pinctada maxima PB - Elsevier SP - 213 KW - cdna KW - pincatda AU - Kono, M AU - Hayashi, N AU - Samata, T PY - 2000/03// UR - http://dx.doi.org/10.1006/bbrc.2000.2274 ER -