Identification of proteins separated by one-dimensional sodium dodecyl sulfate/polyacrylamide gel electrophoresis with matrix-assisted laser desorption/ionization ion trap mass spectrometry; comparison with matrix-assisted laser desorption/ionization time-of-flight mass fingerprinting

@article{citeulike:3169700, abstract = {Digests from ten gel bands containing low abundance proteins were analyzed by both matrix-assisted laser desorption/ionization ion trap (MALDI-IT) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) methods. MALDI-TOF techniques were able to identify only one protein from all 10 gel bands, while MALDI-IT identified eight proteins from the same 10 bands. The ability to perform MS/MS experiments with a MALDI-IT instrument leads to protein identifications based on both peptide molecular mass and sequence information, and is much less prone to errors and uncertainties introduced by peptide fingerprinting methodologies in which protein identification is based on peptide molecular masses alone. Copyright {\copyright} 2003 John Wiley \& Sons, Ltd.}, address = {Research Center for Proteome Analysis, Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 YueYang Road, Shanghai 200031, China; Biospect Inc., 951 Gateway Blvd., South San Francisco, CA 94080, USA; ThermoFinnigan Corp., 355 River Oaks Parkway, San Jose, CA 95134, USA}, author = {Zeng, Rong and Chen, Yong-Bing and Shao, Xiao-Xia and Shieh, Chia-Hui P. and Miller, Ken and Tran, Helen and Xia, Qi-Chang}, citeulike-article-id = {3169700}, doi = {10.1002/rcm.1143}, journal = {Rapid Communications in Mass Spectrometry}, keywords = {maldi}, number = {17}, pages = {1995--2004}, posted-at = {2008-08-28 14:24:29}, priority = {2}, title = {Identification of proteins separated by one-dimensional sodium dodecyl sulfate/polyacrylamide gel electrophoresis with matrix-assisted laser desorption/ionization ion trap mass spectrometry; comparison with matrix-assisted laser desorption/ionization time-of-flight mass fingerprinting}, url = {http://dx.doi.org/10.1002/rcm.1143}, volume = {17}, year = {2003} }

TY - JOUR ID - citeulike:3169700 L3 - citeulike-article-id:3169700 N2 - Digests from ten gel bands containing low abundance proteins were analyzed by both matrix-assisted laser desorption/ionization ion trap (MALDI-IT) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) methods. MALDI-TOF techniques were able to identify only one protein from all 10 gel bands, while MALDI-IT identified eight proteins from the same 10 bands. The ability to perform MS/MS experiments with a MALDI-IT instrument leads to protein identifications based on both peptide molecular mass and sequence information, and is much less prone to errors and uncertainties introduced by peptide fingerprinting methodologies in which protein identification is based on peptide molecular masses alone. Copyright © 2003 John Wiley & Sons, Ltd. IS - 17 JF - Rapid Communications in Mass Spectrometry AD - Research Center for Proteome Analysis, Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 YueYang Road, Shanghai 200031, China; Biospect Inc., 951 Gateway Blvd., South San Francisco, CA 94080, USA; ThermoFinnigan Corp., 355 River Oaks Parkway, San Jose, CA 95134, USA EP - 2004 TI - Identification of proteins separated by one-dimensional sodium dodecyl sulfate/polyacrylamide gel electrophoresis with matrix-assisted laser desorption/ionization ion trap mass spectrometry; comparison with matrix-assisted laser desorption/ionization time-of-flight mass fingerprinting VL - 17 SP - 1995 KW - maldi AU - Zeng, Rong AU - Chen, Yong-Bing AU - Shao, Xiao-Xia AU - Shieh, Chia-Hui AU - Miller, Ken AU - Tran, Helen AU - Xia, Qi-Chang PY - 2003/// UR - http://dx.doi.org/10.1002/rcm.1143 ER -