The carbonic anhydrase domain protein nacrein is expressed in the epithelial cells of the mantle and acts as a negative regulator in calcification in the mollusc Pinctada fucata. Export

@article{citeulike:5414215, abstract = {Signals and organic matrix proteins secreted from the mantle are critical for the development of shells in molluscs. Nacrein, which is composed of a carbonic anhydrase domain and a Gly-X-Asn repeat domain, is one of the organic matrix proteins that accumulates in shells. In situ hybridization revealed that nacrein was expressed in the outer epithelial cells of the mantle of the pearl oyster Pinctada fucata. The recombinant nacrein protein inhibited the precipitation of calcium carbonate from a saturated solution containing CaCl2 and NaHCO3, indicating that it can act as a negative regulator for calcification in the shells of molluscs. Because deletion of the Gly-X-Asn repeat domain of nacrein had a significant effect on the ability of nacrein to inhibit the precipitation of calcium carbonate, it is conceivable that the repeat domain has a primary role in the inhibitory function of nacrein in shell formation. Together these studies suggest that nacrein functions as a negative regulator in calcification in the extrapallial space between the shell and the mantle by inhibiting the precipitation of CaCO3.}, author = {Miyamoto, Hiroshi and Miyoshi, Fumiko and Kohno, Jun}, citeulike-article-id = {5414215}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/15795493}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=15795493}, issn = {0289-0003}, journal = {Zoological science}, keywords = {nacre, protein}, month = {March}, number = {3}, pages = {311--315}, posted-at = {2009-08-11 08:58:00}, priority = {2}, title = {The carbonic anhydrase domain protein nacrein is expressed in the epithelial cells of the mantle and acts as a negative regulator in calcification in the mollusc Pinctada fucata.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/15795493}, volume = {22}, year = {2005} }

TY - JOUR ID - citeulike:5414215 L3 - citeulike-article-id:5414215 N2 - Signals and organic matrix proteins secreted from the mantle are critical for the development of shells in molluscs. Nacrein, which is composed of a carbonic anhydrase domain and a Gly-X-Asn repeat domain, is one of the organic matrix proteins that accumulates in shells. In situ hybridization revealed that nacrein was expressed in the outer epithelial cells of the mantle of the pearl oyster Pinctada fucata. The recombinant nacrein protein inhibited the precipitation of calcium carbonate from a saturated solution containing CaCl2 and NaHCO3, indicating that it can act as a negative regulator for calcification in the shells of molluscs. Because deletion of the Gly-X-Asn repeat domain of nacrein had a significant effect on the ability of nacrein to inhibit the precipitation of calcium carbonate, it is conceivable that the repeat domain has a primary role in the inhibitory function of nacrein in shell formation. Together these studies suggest that nacrein functions as a negative regulator in calcification in the extrapallial space between the shell and the mantle by inhibiting the precipitation of CaCO3. IS - 3 JF - Zoological science SN - 0289-0003 EP - 315 TI - The carbonic anhydrase domain protein nacrein is expressed in the epithelial cells of the mantle and acts as a negative regulator in calcification in the mollusc Pinctada fucata. VL - 22 SP - 311 KW - nacre KW - protein AU - Miyamoto, Hiroshi AU - Miyoshi, Fumiko AU - Kohno, Jun PY - 2005/03// UR - http://view.ncbi.nlm.nih.gov/pubmed/15795493 ER -