Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Export

@article{citeulike:681782, abstract = {A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction. The enzyme, isolated from Thermus aquaticus, greatly simplifies the procedure and, by enabling the amplification reaction to be performed at higher temperatures, significantly improves the specificity, yield, sensitivity, and length of products that can be amplified. Single-copy genomic sequences were amplified by a factor of more than 10 million with very high specificity, and DNA segments up to 2000 base pairs were readily amplified. In addition, the method was used to amplify and detect a target DNA molecule present only once in a sample of 10(5) cells.}, address = {Cetus Corporation, Department of Human Genetics, Emeryville, CA 94608.}, author = {Saiki, R. K. and Gelfand, D. H. and Stoffel, S. and Scharf, S. J. and Higuchi, R. and Horn, G. T. and Mullis, K. B. and Erlich, H. A.}, citeulike-article-id = {681782}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.2448875}, citeulike-linkout-1 = {http://www.sciencemag.org/content/239/4839/487.abstract}, citeulike-linkout-2 = {http://www.sciencemag.org/content/239/4839/487.full.pdf}, citeulike-linkout-3 = {http://www.sciencemag.org/cgi/content/abstract/239/4839/487}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/2448875}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=2448875}, day = {29}, doi = {10.1126/science.2448875}, issn = {0036-8075}, journal = {Science (New York, N.Y.)}, keywords = {pcr}, month = {January}, number = {4839}, pages = {487--491}, posted-at = {2008-06-12 10:01:14}, priority = {2}, title = {Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.}, url = {http://dx.doi.org/10.1126/science.2448875}, volume = {239}, year = {1988} }

TY - JOUR ID - citeulike:681782 L3 - citeulike-article-id:681782 N2 - A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction. The enzyme, isolated from Thermus aquaticus, greatly simplifies the procedure and, by enabling the amplification reaction to be performed at higher temperatures, significantly improves the specificity, yield, sensitivity, and length of products that can be amplified. Single-copy genomic sequences were amplified by a factor of more than 10 million with very high specificity, and DNA segments up to 2000 base pairs were readily amplified. In addition, the method was used to amplify and detect a target DNA molecule present only once in a sample of 10(5) cells. IS - 4839 JF - Science (New York, N.Y.) SN - 0036-8075 AD - Cetus Corporation, Department of Human Genetics, Emeryville, CA 94608. EP - 491 TI - Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. VL - 239 SP - 487 KW - pcr AU - Saiki, RK AU - Gelfand, DH AU - Stoffel, S AU - Scharf, SJ AU - Higuchi, R AU - Horn, GT AU - Mullis, KB AU - Erlich, HA PY - 1988/01/29/ UR - http://dx.doi.org/10.1126/science.2448875 ER -