Normalization strategies for cDNA microarrays. Export

@article{Schuchhardt2000, abstract = {Multiple Arabidopsis thaliana clones from an experimental series of cDNA microarrays are evaluated in order to identify essential sources of noise in the spotting and hybridization process. Theoretical and experimental strategies for an improved quantitative evaluation of cDNA microarrays are proposed and tested on a series of differently diluted control clones. Several sources of noise are identified from the data. Systematic and stochastic fluctuations in the spotting process are reduced by control spots and statistical techniques. The reliability of slide to slide comparison is critically assessed within the statistical framework of pattern matching and classification.}, address = {Institute for Theoretical Biology, Humboldt-Universit\"{a}t zu Berlin, Invalidenstrasse 43, D-10115 Berlin, Germany. jo@itb.biologie.hu-berlin.de}, author = {Schuchhardt, J. and Beule, D. and Malik, A. and Wolski, E. and Eickhoff, H. and Lehrach, H. and Herzel, H.}, citeulike-article-id = {241032}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/nar/28.10.e47}, citeulike-linkout-1 = {http://nar.oxfordjournals.org/cgi/content/abstract/28/10/e47}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/10773095}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=10773095}, day = {15}, doi = {10.1093/nar/28.10.e47}, issn = {1362-4962}, journal = {Nucleic Acids Res}, keywords = {microarray, tvcv}, month = {May}, number = {10}, posted-at = {2008-01-24 15:15:59}, priority = {2}, title = {Normalization strategies for cDNA microarrays.}, url = {http://dx.doi.org/10.1093/nar/28.10.e47}, volume = {28}, year = {2000} }

TY - JOUR ID - Schuchhardt2000 L3 - citeulike-article-id:241032 N2 - Multiple Arabidopsis thaliana clones from an experimental series of cDNA microarrays are evaluated in order to identify essential sources of noise in the spotting and hybridization process. Theoretical and experimental strategies for an improved quantitative evaluation of cDNA microarrays are proposed and tested on a series of differently diluted control clones. Several sources of noise are identified from the data. Systematic and stochastic fluctuations in the spotting process are reduced by control spots and statistical techniques. The reliability of slide to slide comparison is critically assessed within the statistical framework of pattern matching and classification. IS - 10 JF - Nucleic Acids Res SN - 1362-4962 AD - Institute for Theoretical Biology, Humboldt-Universität zu Berlin, Invalidenstrasse 43, D-10115 Berlin, Germany. jo@itb.biologie.hu-berlin.de TI - Normalization strategies for cDNA microarrays. VL - 28 KW - microarray KW - tvcv AU - Schuchhardt, J AU - Beule, D AU - Malik, A AU - Wolski, E AU - Eickhoff, H AU - Lehrach, H AU - Herzel, H PY - 2000/05/15/ UR - http://dx.doi.org/10.1093/nar/28.10.e47 ER -