Artificial gynogenesis with ultraviolet light-irradiated sperm in the Pacific oyster, Crassostrea gigas. I. Induction and survival Export

@article{citeulike:3801588, abstract = {Cytochalasin B (CB) treatment applied during meiosis II blocked the release of the second polar body in 83\% of the gynogenetically activated eggs. Following diploidization, 18\% of the activated eggs survived to D-stage. Although the post-D-stage survival was low, viable gynogenetic diploids were obtained. CB treatment applied to block both polar body I and II produced high levels of gynogenetic tetraploids (95\%) which were not detected at 2 months post-fertilization. The use of inbred females did not improve the survival of gynogenetic animals in this study. The low survival of the gynogenetic animals was probably caused by genetic damage resulting from UV irradiation of sperm.}, author = {Guo, X.}, citeulike-article-id = {3801588}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/0044-8486(93)90474-D}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/0044-8486(93)90474-D}, day = {15}, doi = {10.1016/0044-8486(93)90474-D}, issn = {00448486}, journal = {Aquaculture}, keywords = {gigas, gynogenesis, mollusc, oyster, polyploidy, tetraploid, zzp}, month = {June}, number = {3}, pages = {201--214}, posted-at = {2008-12-17 15:05:36}, priority = {5}, title = {Artificial gynogenesis with ultraviolet light-irradiated sperm in the Pacific oyster, Crassostrea gigas. I. Induction and survival}, url = {http://dx.doi.org/10.1016/0044-8486(93)90474-D}, volume = {113}, year = {1993} }

TY - JOUR ID - citeulike:3801588 L3 - citeulike-article-id:3801588 N2 - Cytochalasin B (CB) treatment applied during meiosis II blocked the release of the second polar body in 83% of the gynogenetically activated eggs. Following diploidization, 18% of the activated eggs survived to D-stage. Although the post-D-stage survival was low, viable gynogenetic diploids were obtained. CB treatment applied to block both polar body I and II produced high levels of gynogenetic tetraploids (95%) which were not detected at 2 months post-fertilization. The use of inbred females did not improve the survival of gynogenetic animals in this study. The low survival of the gynogenetic animals was probably caused by genetic damage resulting from UV irradiation of sperm. IS - 3 JF - Aquaculture SN - 00448486 EP - 214 TI - Artificial gynogenesis with ultraviolet light-irradiated sperm in the Pacific oyster, Crassostrea gigas. I. Induction and survival VL - 113 SP - 201 KW - gigas KW - gynogenesis KW - mollusc KW - oyster KW - polyploidy KW - tetraploid KW - zzp AU - Guo, X PY - 1993/06/15/ UR - http://dx.doi.org/10.1016/0044-8486(93)90474-D ER -