A genetically encoded fluorescent sensor of ERK activity Export

@article{citeulike:3767158, abstract = {10.1073/pnas.0804598105 The activity of the ERK has complex spatial and temporal dynamics that are important for the specificity of downstream effects. However, current biochemical techniques do not allow for the measurement of ERK signaling with fine spatiotemporal resolution. We developed a genetically encoded, FRET-based sensor of ERK activity (the extracellular signal-regulated kinase activity reporter, EKAR), optimized for signal-to-noise ratio and fluorescence lifetime imaging. EKAR selectively and reversibly reported ERK activation in HEK293 cells after epidermal growth factor stimulation. EKAR signals were correlated with ERK phosphorylation, required ERK activity, and did not report the activities of JNK or p38. EKAR reported ERK activation in the dendrites and nucleus of hippocampal pyramidal neurons in brain slices after theta-burst stimuli or trains of back-propagating action potentials. EKAR therefore permits the measurement of spatiotemporal ERK signaling dynamics in living cells, including in neuronal compartments in intact tissues.}, author = {Harvey, Christopher D. and Ehrhardt, Anka G. and Cellurale, Cristina and Zhong, Haining and Yasuda, Ryohei and Davis, Roger J. and Svoboda, Karel}, citeulike-article-id = {3767158}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0804598105}, citeulike-linkout-1 = {http://www.pnas.org/content/105/49/19264.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/105/49/19264.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19033456}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19033456}, day = {9}, doi = {10.1073/pnas.0804598105}, issn = {1091-6490}, journal = {Proceedings of the National Academy of Sciences}, keywords = {erk, fluorescent, sensor}, month = {December}, number = {49}, pages = {19264--19269}, posted-at = {2009-10-15 17:34:15}, priority = {2}, title = {A genetically encoded fluorescent sensor of ERK activity}, url = {http://dx.doi.org/10.1073/pnas.0804598105}, volume = {105}, year = {2008} }

TY - JOUR ID - citeulike:3767158 L3 - citeulike-article-id:3767158 N2 - 10.1073/pnas.0804598105 The activity of the ERK has complex spatial and temporal dynamics that are important for the specificity of downstream effects. However, current biochemical techniques do not allow for the measurement of ERK signaling with fine spatiotemporal resolution. We developed a genetically encoded, FRET-based sensor of ERK activity (the extracellular signal-regulated kinase activity reporter, EKAR), optimized for signal-to-noise ratio and fluorescence lifetime imaging. EKAR selectively and reversibly reported ERK activation in HEK293 cells after epidermal growth factor stimulation. EKAR signals were correlated with ERK phosphorylation, required ERK activity, and did not report the activities of JNK or p38. EKAR reported ERK activation in the dendrites and nucleus of hippocampal pyramidal neurons in brain slices after theta-burst stimuli or trains of back-propagating action potentials. EKAR therefore permits the measurement of spatiotemporal ERK signaling dynamics in living cells, including in neuronal compartments in intact tissues. IS - 49 JF - Proceedings of the National Academy of Sciences SN - 1091-6490 EP - 19269 TI - A genetically encoded fluorescent sensor of ERK activity VL - 105 SP - 19264 KW - erk KW - fluorescent KW - sensor AU - Harvey, Christopher AU - Ehrhardt, Anka AU - Cellurale, Cristina AU - Zhong, Haining AU - Yasuda, Ryohei AU - Davis, Roger AU - Svoboda, Karel PY - 2008/12/09/ UR - http://dx.doi.org/10.1073/pnas.0804598105 ER -