Mapping long-range chromatin organization within the chicken alpha-globin gene domain using oligonucleotide DNA arrays. Export

@article{citeulike:326761, abstract = {We have analyzed the organization of the chicken alpha-globin gene domain using DNA miniarrays and have found two novel chromatin loop attachment regions. We have found a 40-kb loop domain that includes all the alpha-globin genes in cells of erythroid origin. One of the domain borders colocalizes almost exactly with a strong MAR element and with a block of enhancer-blocking elements found earlier at the upstream end of the alpha-globin gene domain. The domain structure was found to be different in a lymphoid cell line DT40. We propose to use the technique of DNA arrays to map the nuclear matrix attachment sites that define the borders of chromosome loop domains. The technique of DNA arrays permits a large number of DNA sequences to be immobilized on a glass or nylon matrix. This may prove useful for mapping chromatin loop positions within the human genome by using a pool of chromatin loop attachment regions as a probe in a hybridization with a DNA chip containing a specific DNA region.}, address = {Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.}, author = {Ioudinkova, E. and Petrov, A. and Razin, S. V. and Vassetzky, Y. S.}, citeulike-article-id = {326761}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.ygeno.2004.09.008}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15607430}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15607430}, citeulike-linkout-3 = {http://www.sciencedirect.com/science/article/B6WG1-4DTKB9R-1/2/5e079046fe28ef9cf62e41447ebf19f6}, doi = {10.1016/j.ygeno.2004.09.008}, issn = {0888-7543}, journal = {Genomics}, keywords = {alpha\_globin, chromatin, dea, organization, puces\_adn}, month = {January}, number = {1}, pages = {143--151}, posted-at = {2005-09-20 08:54:10}, priority = {0}, title = {Mapping long-range chromatin organization within the chicken alpha-globin gene domain using oligonucleotide DNA arrays.}, url = {http://dx.doi.org/10.1016/j.ygeno.2004.09.008}, volume = {85}, year = {2005} }

TY - JOUR ID - citeulike:326761 L3 - citeulike-article-id:326761 N2 - We have analyzed the organization of the chicken alpha-globin gene domain using DNA miniarrays and have found two novel chromatin loop attachment regions. We have found a 40-kb loop domain that includes all the alpha-globin genes in cells of erythroid origin. One of the domain borders colocalizes almost exactly with a strong MAR element and with a block of enhancer-blocking elements found earlier at the upstream end of the alpha-globin gene domain. The domain structure was found to be different in a lymphoid cell line DT40. We propose to use the technique of DNA arrays to map the nuclear matrix attachment sites that define the borders of chromosome loop domains. The technique of DNA arrays permits a large number of DNA sequences to be immobilized on a glass or nylon matrix. This may prove useful for mapping chromatin loop positions within the human genome by using a pool of chromatin loop attachment regions as a probe in a hybridization with a DNA chip containing a specific DNA region. IS - 1 JF - Genomics SN - 0888-7543 AD - Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia. EP - 151 TI - Mapping long-range chromatin organization within the chicken alpha-globin gene domain using oligonucleotide DNA arrays. VL - 85 SP - 143 KW - alpha_globin KW - chromatin KW - dea KW - organization KW - puces_adn AU - Ioudinkova, E AU - Petrov, A AU - Razin, SV AU - Vassetzky, YS PY - 2005/01// UR - http://dx.doi.org/10.1016/j.ygeno.2004.09.008 ER -