A genetic mosaic screen of essential zygotic genes in Caenorhabditis elegans. Export

@article{citeulike:3275813, abstract = {We have devised a simple genetic mosaic screen, which circumvents the difficulties posed by phenotypic analysis of early lethal mutants, to analyze essential zygotic genes in Caenorhabditis elegans. The screen attempts to distinguish genes involved in cell type and/or lineage specific processes such as determination, differentiation or morphogenesis from genes involved in general processes such as intermediary metabolism by using the pattern of gene function to classify genes: genes required in one or a subset of early blastomeres may have specific functions, whereas genes required in all early blastomeres may have general functions. We found that 12 of 17 genes examined function in specific early blastomeres, suggesting that many zygotic genes contribute to specific early processes. We discuss the advantages and limitations of this screen, which is applicable to other regions of the C. elegans genome.}, author = {Bucher, E. A. and Greenwald, I. S.}, citeulike-article-id = {3275813}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/2071016}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=2071016}, citeulike-linkout-2 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00001406}, journal = {Genetics}, keywords = {alleles, article, c\_elegans, caenorhabditis\_cytology, caenorhabditis\_elegans, caenorhabditis\_embryology, caenorhabditis\_genetics, caenorhabditis\_growth\_and\_development, celegans, cell\_division, elegans, genes\_lethal, genes\_reiterated, genetic\_complementation\_test, genetic\_techniques, mosaicism\_genetics, mutagenesis, nematode, phenotype, wormbase, zygote}, pages = {281--292}, posted-at = {2008-09-16 21:04:33}, priority = {3}, title = {A genetic mosaic screen of essential zygotic genes in Caenorhabditis elegans.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/2071016}, volume = {128}, year = {1991} }

TY - JOUR ID - citeulike:3275813 L3 - citeulike-article-id:3275813 N2 - We have devised a simple genetic mosaic screen, which circumvents the difficulties posed by phenotypic analysis of early lethal mutants, to analyze essential zygotic genes in Caenorhabditis elegans. The screen attempts to distinguish genes involved in cell type and/or lineage specific processes such as determination, differentiation or morphogenesis from genes involved in general processes such as intermediary metabolism by using the pattern of gene function to classify genes: genes required in one or a subset of early blastomeres may have specific functions, whereas genes required in all early blastomeres may have general functions. We found that 12 of 17 genes examined function in specific early blastomeres, suggesting that many zygotic genes contribute to specific early processes. We discuss the advantages and limitations of this screen, which is applicable to other regions of the C. elegans genome. JF - Genetics EP - 292 TI - A genetic mosaic screen of essential zygotic genes in Caenorhabditis elegans. VL - 128 SP - 281 KW - alleles KW - article KW - c_elegans KW - caenorhabditis_cytology KW - caenorhabditis_elegans KW - caenorhabditis_embryology KW - caenorhabditis_genetics KW - caenorhabditis_growth_and_development KW - celegans KW - cell_division KW - elegans KW - genes_lethal KW - genes_reiterated KW - genetic_complementation_test KW - genetic_techniques KW - mosaicism_genetics KW - mutagenesis KW - nematode KW - phenotype KW - wormbase KW - zygote AU - Bucher, EA AU - Greenwald, IS PY - 1991/// UR - http://view.ncbi.nlm.nih.gov/pubmed/2071016 ER -