An acid phosphatase as a biochemical marker for intestinal development in the nematode Caenorhabditis elegans. Export

@article{citeulike:3275870, abstract = {We describe an acid phosphatase enzyme (EC 3.1.3.2) that is localized to the intestine of the nematode Caenorhabditis elegans and that should serve as a convenient biochemical marker for gut differentiation. In adult worms, acid phosphatase activity is located along the edge of the gut lumen in the vicinity of the intestinal brush border. All but the anterior six cells of the intestine stain for phosphatase activity; the nonstaining cells all descend from the Ea(l/r)(a/p)a cells. Acid phosphatase activity is low in oocytes and early embryos but increases substantially when embryos reach late morphogenesis stage; this increase corresponds to the appearance of a major band of acid phosphatase activity detectable on isoelectric focusing gels. We designate this band as the product of the pho-1 gene. The pattern of acid phosphatase expression in several embryonic mutants suggests that pho-1 expression in the developing intestine is lineage autonomous. We induced an isoelectric focusing variant in the pho-1 enzyme and used this to map the pho-1 locus about 1.5 map units to the left of center of chromosome II. We purified the pho-1 enzyme to homogeneity (6500-fold purification; 4\% recovery of activity); the pho-1 acid phosphatase is a homodimeric glycoprotein with a subunit molecular weight of 55,000 Da. This paper establishes a new experimental system with which to investigate the molecular basis of lineage-specific gene expression during C. elegans development.}, author = {Beh, C. T. and Ferrari, D. C. and Chung, M. A. and Mcghee, J. D.}, citeulike-article-id = {3275870}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/1652526}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=1652526}, citeulike-linkout-2 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00001454}, journal = {Developmental Biology}, keywords = {acid\_phosphatase\_genetics, acid\_phosphatase\_isolation\_and\_purification, acid\_phosphatase\_metabolism, article, biological\_markers, c\_elegans, caenorhabditis\_elegans, caenorhabditis\_embryology, caenorhabditis\_enzymology, caenorhabditis\_growth\_and\_development, celegans, chromatography, chromatography\_affinity, chromatography\_gel, chromatography\_ion\_exchange, elegans, embryo\_non\_mammalian\_enzymology, embryo\_non\_mammalian\_physiology, histocytochemistry, hydroxyapatites, intestines\_embryology, intestines\_enzymology, intestines\_growth\_and\_development, isoelectric\_focusing, kinetics, nematode, wormbase}, pages = {133--143}, posted-at = {2008-09-16 21:16:20}, priority = {3}, title = {An acid phosphatase as a biochemical marker for intestinal development in the nematode Caenorhabditis elegans.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/1652526}, volume = {147}, year = {1991} }

TY - JOUR ID - citeulike:3275870 L3 - citeulike-article-id:3275870 N2 - We describe an acid phosphatase enzyme (EC 3.1.3.2) that is localized to the intestine of the nematode Caenorhabditis elegans and that should serve as a convenient biochemical marker for gut differentiation. In adult worms, acid phosphatase activity is located along the edge of the gut lumen in the vicinity of the intestinal brush border. All but the anterior six cells of the intestine stain for phosphatase activity; the nonstaining cells all descend from the Ea(l/r)(a/p)a cells. Acid phosphatase activity is low in oocytes and early embryos but increases substantially when embryos reach late morphogenesis stage; this increase corresponds to the appearance of a major band of acid phosphatase activity detectable on isoelectric focusing gels. We designate this band as the product of the pho-1 gene. The pattern of acid phosphatase expression in several embryonic mutants suggests that pho-1 expression in the developing intestine is lineage autonomous. We induced an isoelectric focusing variant in the pho-1 enzyme and used this to map the pho-1 locus about 1.5 map units to the left of center of chromosome II. We purified the pho-1 enzyme to homogeneity (6500-fold purification; 4% recovery of activity); the pho-1 acid phosphatase is a homodimeric glycoprotein with a subunit molecular weight of 55,000 Da. This paper establishes a new experimental system with which to investigate the molecular basis of lineage-specific gene expression during C. elegans development. JF - Developmental Biology EP - 143 TI - An acid phosphatase as a biochemical marker for intestinal development in the nematode Caenorhabditis elegans. VL - 147 SP - 133 KW - acid_phosphatase_genetics KW - acid_phosphatase_isolation_and_purification KW - acid_phosphatase_metabolism KW - article KW - biological_markers KW - c_elegans KW - caenorhabditis_elegans KW - caenorhabditis_embryology KW - caenorhabditis_enzymology KW - caenorhabditis_growth_and_development KW - celegans KW - chromatography KW - chromatography_affinity KW - chromatography_gel KW - chromatography_ion_exchange KW - elegans KW - embryo_non_mammalian_enzymology KW - embryo_non_mammalian_physiology KW - histocytochemistry KW - hydroxyapatites KW - intestines_embryology KW - intestines_enzymology KW - intestines_growth_and_development KW - isoelectric_focusing KW - kinetics KW - nematode KW - wormbase AU - Beh, CT AU - Ferrari, DC AU - Chung, MA AU - Mcghee, JD PY - 1991/// UR - http://view.ncbi.nlm.nih.gov/pubmed/1652526 ER -