A survival pathway for Caenorhabditis elegans with a blocked unfolded protein response. Export

@article{citeulike:3283364, abstract = {The unfolded protein response (UPR) counteracts stress caused by unprocessed ER client proteins. A genome-wide survey showed impaired induction of many UPR target genes in xbp-1 mutant Caenorhabditis elegans that are unable to signal in the highly conserved IRE1-dependent UPR pathway. However a family of genes, abu (activated in blocked UPR), was induced to higher levels in ER-stressed xbp-1 mutant animals than in ER-stressed wild-type animals. RNA-mediated interference (RNAi) inactivation of a representative abu family member, abu-1 (AC3.3), activated the ER stress marker hsp-4:.gfp in otherwise normal animals and killed 50\% of ER-stressed ire-1 and xbp-1 mutant animals. Abu-1(RNAi) also enhanced the effect of inactivation of sel-1, an ER-associated protein degradation gene. The nine abu genes encode highly related type I transmembrane proteins whose lumenal domains have sequence similarity to a mammalian cell surface scavenger receptor of endothelial cells that binds chemically modified extracellular proteins and directs their lysosomal degradation. Our findings that ABU-1 is an intracellular protein located within the endomembrane system that is induced by ER stress in xbp-1 mutant animals suggest that ABU proteins may interact with abnormal ER client proteins and this function may be particularly important in animals with an impaired UPR.}, author = {Urano, F. and Calfon, M. and Yoneda, T. and Yun, C. and Kiraly, M. and Clark, S. G. and Ron, D.}, citeulike-article-id = {3283364}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/12186849}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=12186849}, citeulike-linkout-2 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00005432}, journal = {Journal of Cell Biology}, keywords = {abu-1, ac33, article, c41c44, c\_elegans, caenorhabditis\_elegans, celegans, elegans, f45d35, ire-1, nematode, r743, sel-1, wormbase, xbp-1}, pages = {639--646}, posted-at = {2008-09-18 02:22:11}, priority = {3}, title = {A survival pathway for Caenorhabditis elegans with a blocked unfolded protein response.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/12186849}, volume = {158}, year = {2002} }

TY - JOUR ID - citeulike:3283364 L3 - citeulike-article-id:3283364 N2 - The unfolded protein response (UPR) counteracts stress caused by unprocessed ER client proteins. A genome-wide survey showed impaired induction of many UPR target genes in xbp-1 mutant Caenorhabditis elegans that are unable to signal in the highly conserved IRE1-dependent UPR pathway. However a family of genes, abu (activated in blocked UPR), was induced to higher levels in ER-stressed xbp-1 mutant animals than in ER-stressed wild-type animals. RNA-mediated interference (RNAi) inactivation of a representative abu family member, abu-1 (AC3.3), activated the ER stress marker hsp-4:.gfp in otherwise normal animals and killed 50% of ER-stressed ire-1 and xbp-1 mutant animals. Abu-1(RNAi) also enhanced the effect of inactivation of sel-1, an ER-associated protein degradation gene. The nine abu genes encode highly related type I transmembrane proteins whose lumenal domains have sequence similarity to a mammalian cell surface scavenger receptor of endothelial cells that binds chemically modified extracellular proteins and directs their lysosomal degradation. Our findings that ABU-1 is an intracellular protein located within the endomembrane system that is induced by ER stress in xbp-1 mutant animals suggest that ABU proteins may interact with abnormal ER client proteins and this function may be particularly important in animals with an impaired UPR. JF - Journal of Cell Biology EP - 646 TI - A survival pathway for Caenorhabditis elegans with a blocked unfolded protein response. VL - 158 SP - 639 KW - abu-1 KW - ac33 KW - article KW - c41c44 KW - c_elegans KW - caenorhabditis_elegans KW - celegans KW - elegans KW - f45d35 KW - ire-1 KW - nematode KW - r743 KW - sel-1 KW - wormbase KW - xbp-1 AU - Urano, F AU - Calfon, M AU - Yoneda, T AU - Yun, C AU - Kiraly, M AU - Clark, SG AU - Ron, D PY - 2002/// UR - http://view.ncbi.nlm.nih.gov/pubmed/12186849 ER -