PROGRESS REPORT ON TWO GENES THAT INTERACT WITH LIN-12: SEL-9 AND SUP-17 Export

@inproceedings{citeulike:3312417, abstract = {Two sel-9 mutations were originally isolated as suppressors of a lin-12 hypomorphic allele (Sundaram and Greenwald, 1993), and we have isolated 6 additional alleles of sel-9 by a noncomplementation screen. We have examined the genetic interactions between sel-9 and lin-12 alleles. None of the alleles of sel-9 behave like sDf47, a deficiency of the locus(Hunter and Wood, 1992). These results indicate either that none of the sel-9 alleles are null alleles, or that the deficiency does not behave like a null allele (perhaps because it reduces the activity of another interacting gene). The complex genetics have made sel-9 difficult to clone, and we will present the status of our efforts at the meeting. sup-17 was identified by suppressors of lin-12(d) alleles (Ferguson and Horvitz, 1985; F. Tax, J. Thomas, E. Ferguson and H.R. Horvitz, 1997). We have been investigating interactions of sup-17 with lin-12 and glp-1 hypomorphs, and with other genes that interact with lin-12. We have also begun to try to isolate sup-17, beginning with a 180kb YAC clone that Mark Metzstein (personal communication) used to rescue sup-17 in the process of correlating the genetic and physical maps in the ces-1 region. We have used in vivo recombination in yeast to truncate this YAC clone, and have identified a 60 kb region that is able to rescue sup-17. We hope to have made additional progress towards cloning sup-17 by the meeting.}, author = {C-H, Wen and Greenwald, I. S.}, citeulike-article-id = {3312417}, citeulike-linkout-0 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00022802}, journal = {International C. elegans Meeting}, keywords = {c\_elegans, caenorhabditis\_elegans, celegans, ces-1, dy37, elegans, f02a96, f43g911, glp-1, lin-12, meeting\_abstract, nematode, r1078, sel-9, sup-17, w02d77, wormbase}, posted-at = {2008-09-22 01:12:45}, priority = {3}, title = {PROGRESS REPORT ON TWO GENES THAT INTERACT WITH LIN-12: SEL-9 AND SUP-17}, url = {http://www.wormbase.org/db/misc/paper?name=WBPaper00022802}, year = {1997} }

TY - CONF ID - citeulike:3312417 L3 - citeulike-article-id:3312417 N2 - Two sel-9 mutations were originally isolated as suppressors of a lin-12 hypomorphic allele (Sundaram and Greenwald, 1993), and we have isolated 6 additional alleles of sel-9 by a noncomplementation screen. We have examined the genetic interactions between sel-9 and lin-12 alleles. None of the alleles of sel-9 behave like sDf47, a deficiency of the locus(Hunter and Wood, 1992). These results indicate either that none of the sel-9 alleles are null alleles, or that the deficiency does not behave like a null allele (perhaps because it reduces the activity of another interacting gene). The complex genetics have made sel-9 difficult to clone, and we will present the status of our efforts at the meeting. sup-17 was identified by suppressors of lin-12(d) alleles (Ferguson and Horvitz, 1985; F. Tax, J. Thomas, E. Ferguson and H.R. Horvitz, 1997). We have been investigating interactions of sup-17 with lin-12 and glp-1 hypomorphs, and with other genes that interact with lin-12. We have also begun to try to isolate sup-17, beginning with a 180kb YAC clone that Mark Metzstein (personal communication) used to rescue sup-17 in the process of correlating the genetic and physical maps in the ces-1 region. We have used in vivo recombination in yeast to truncate this YAC clone, and have identified a 60 kb region that is able to rescue sup-17. We hope to have made additional progress towards cloning sup-17 by the meeting. JF - International C. elegans Meeting TI - PROGRESS REPORT ON TWO GENES THAT INTERACT WITH LIN-12: SEL-9 AND SUP-17 KW - c_elegans KW - caenorhabditis_elegans KW - celegans KW - ces-1 KW - dy37 KW - elegans KW - f02a96 KW - f43g911 KW - glp-1 KW - lin-12 KW - meeting_abstract KW - nematode KW - r1078 KW - sel-9 KW - sup-17 KW - w02d77 KW - wormbase AU - C-H, Wen AU - Greenwald, IS PY - 1997/// UR - http://www.wormbase.org/db/misc/paper?name=WBPaper00022802 ER -